Conductive Turbulence Grid for Cell Substance Introduction
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Solution Overview
Problem
Physical methods for introducing substances into cells, such as electroporation, face limitations in introduction efficiency and cytotoxicity compared to biological and biochemical methods.
Innovation Solution
A substance introduction device and method utilizing a turbulence grid formed of conductive material that applies charge and stress to cells in a fluid, allowing adjustable flow rates and charge intensities to enhance introduction efficiency while minimizing cytotoxicity.
Engineering Contradictions & Design Principles
Engineering Contradiction Analysis
1Reliability
If physical introduction methods such as electroporation are used, then reproducibility is improved and residues are reduced, but introduction efficiency decreases and cytotoxicity increases
Solution Approach 1:
The patent combines turbulence generation and charge application into a single integrated device. The turbulence grid is formed of conductive material that generates both hydrodynamic turbulence and electric charge simultaneously, merging two separate functions (mechanical stress application and electroporation) into one unified system that acts on cells in a single pass
2Loss of substance
If physical introduction methods such as electroporation are used, then residues are reduced, but cytotoxicity increases
Solution Approach 1:
The patent employs adjustable parameters including flow rate control and charge intensity modulation. The turbulence grid structure and electrical charge characteristics can be dynamically adjusted to optimize the balance between substance introduction effectiveness and cell viability, allowing customization for different cell types and substances
3Stability of the object's composition
If turbulence is generated using non-conductive materials, then fluid mixing is improved, but charge application to cells is lost
Solution Approach 1:
The turbulence grid is constructed from conductive material that combines the hydrodynamic properties needed for turbulence generation with electrical conductivity for charge application. This composite functionality allows the same structural element to perform both fluid mixing and electrical charge delivery simultaneously
Applied Scientific Principles
This section explains which scientific principles are used to turn an abstract innovation direction into a practical engineering solution.
Function Achieved in This Case
The device and method improve introduction efficiency and reduce cytotoxicity by simultaneously applying stress and charge through a conductive turbulence grid, optimizing the ratio of conductive material and flow rate for effective substance delivery.
Implementation Method 1
at least one turbulence grid that generates turbulence in a fluid containing a cell and a substance to be introduced into the cell
Implementation Method 2
at least a part of the at least one turbulence grid is formed of a conductive material that applies charge to the cell in the fluid
Data Source
AI summary
A substance introduction device includes at least one turbulence grid that generates turbulence in a fluid containing a cell and a substance to be introduced into the cell, in which at least a part of the at least one turbulence grid is formed of a conductive material that applies charge to the cell in the fluid. A substance introduction method includes a stress/charge applying step of applying, while generating turbulence by flowing a fluid containing a cell and a substance to be introduced into the cell through at least one turbulence grid, charge to the cell in the fluid by a conductive material forming at least a part of the at least one turbulence grid.