Contiguous Overlapping Peptides for House Dust Mite Allergy Treatment

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Solution Overview

Problem

Current treatments for house dust mite allergy, such as specific immunotherapy (SIT), are lengthy and often accompanied by side effects, necessitating a more efficient and safer alternative that can rapidly reduce allergic symptoms.

Innovation Solution

The development of contiguous overlapping peptides (COPs) derived from the major allergens Der p 1 and Der p 2, which include the complete sequence of these allergens, are used to treat house dust mite allergies. These peptides are administered in a shorter regimen, reducing IgE binding and eliciting an immune response without forming the original allergen's three-dimensional structure, thereby minimizing allergic reactions.

Engineering Contradictions & Design Principles

VSEngineering Contradiction Analysis

1Reliability

If traditional specific immunotherapy (SIT) is used to treat house dust mite allergy, then the treatment can induce immune response and reduce allergic symptoms, but the treatment period is lengthy and side effects occur

Engineering Contradiction:
Improveeffectiveness of allergy treatmentVSAvoidtreatment duration
Core Design Contradiction:
ReliabilityVSLoss of time

Solution Approach 1:

The patent segments the major allergens Der p 1 and Der p 2 into multiple contiguous overlapping peptides (COPs). Each peptide covers a specific region of the allergen sequence, and together they comprehensively cover the entire allergen structure. This segmentation allows the immune system to respond to multiple epitopes simultaneously, accelerating the immunotherapy process while maintaining effectiveness and reducing treatment duration.

Inventive Principle:
Principle #1Segmentation

Solution Approach 2:

The patent changes the molecular parameters of the allergens by using truncated peptide sequences instead of full-length proteins. The COPs are designed to cover 70-90% of the allergen sequence while excluding specific regions that trigger strong IgE-mediated allergic reactions. This parameter change enables faster immune response induction with reduced side effects.

Inventive Principle:
Principle #35Parameter changes

2Reliability

If traditional specific immunotherapy (SIT) is used to treat house dust mite allergy, then the treatment can induce immune response and reduce allergic symptoms, but side effects such as allergic reactions occur

Engineering Contradiction:
Improveeffectiveness of allergy treatmentVSAvoidside effects and allergic reactions
Core Design Contradiction:
ReliabilityVSObject-affected harmful factors

Solution Approach 1:

The patent extracts specific regions from the full-length allergen sequences that are responsible for triggering strong IgE-mediated allergic reactions. By designing COPs that cover 70-90% of the allergen sequence while deliberately excluding highly allergenic regions, the treatment maintains immunogenicity while reducing side effects. This extraction approach allows effective allergy treatment with minimized harmful reactions.

Inventive Principle:
Principle #2Taking out (Extraction)

Solution Approach 2:

The patent converts the harmful IgE-mediated allergic response into a beneficial immune response by using COPs that stimulate T-cell mediated immunity rather than IgE production. The truncated peptide sequences are designed to induce protective immune responses (IgG4 antibodies and T-regulatory cell activation) without triggering the harmful IgE pathway, thus converting potential harm into therapeutic benefit.

Inventive Principle:
Principle #22Blessing in disguise (Convert harm into benefit)

3Reliability

If full-length allergens are used in immunotherapy, then complete immune response can be induced, but strong IgE binding and anaphylactic shock risk occur

Engineering Contradiction:
Improvecompleteness of immune responseVSAvoidIgE reactivity and anaphylactic shock
Core Design Contradiction:
ReliabilityVSObject-affected harmful factors

Solution Approach 1:

The patent segments full-length allergens into multiple overlapping peptides, each covering a specific region. This segmentation prevents the formation of complete three-dimensional allergen structures that would bind IgE antibodies, while still presenting multiple linear epitopes to the immune system. The result is comprehensive immune response induction without IgE-mediated anaphylactic shock risk.

Inventive Principle:
Principle #1Segmentation

Solution Approach 2:

The patent changes the structural parameters of allergens by using truncated sequences (70-90% coverage) instead of full-length proteins. This parameter change eliminates the ability of allergens to form their native three-dimensional conformation, thereby preventing IgE binding while maintaining linear epitope recognition by T-cells and B-cells, achieving complete immune response without anaphylaxis risk.

Inventive Principle:
Principle #35Parameter changes

Data Source

PatentEP3003366B1Contiguous overlapping peptides for treatment of house dust mites allergy
Publication Date: 2020.08.05 ANERGIS
  • EP3003366B1 patent drawingFigure 1A~2C
  • EP3003366B1 patent drawingFigure 3A~4B
  • EP3003366B1 patent drawingFigure 5A~7B

AI summary

Contiguous overlapping peptides (COPs) for the treatment of allergic patients by Specific Immunotherapy (SIT) are provided from the sequence of the major allergens of house dust mites Der p 1 and Der p 2. Such peptides while providing all potential T cell epitopes are devoid of the three dimensional structure of the original allergen, therefore reducing their ability to bind IgE. As a result increased amounts of COPs can be administered per injection, therefore reducing both the number of injections and the length of the immunotherapy treatment.