Control Area Generation for Biological Sample Staining Verification
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Solution Overview
Problem
Existing biological sample analysis methods face challenges in efficiently verifying the success of staining procedures, especially when dealing with large collections of samples, as they require rearrangement and dedicated analysis equipment.
Innovation Solution
A method and system for generating a control area on a surface with a control analyte, which can be arranged separately from the biological sample, using a solution deposited as droplets with a polymerizing reagent to create a robust and flexible control area, allowing for contactless deposition and precise placement, enabling verification of staining without rearranging samples.
Engineering Contradictions & Design Principles
Engineering Contradiction Analysis
1Reliability
If biological samples are analyzed using staining procedures, then the spatial distribution of target analytes can be determined, but the staining procedure cannot be efficiently verified without rearranging samples and using dedicated analysis equipment
Solution Approach 1:
The control area is integrated directly onto the sample holder with the biological samples, merging the verification function with the sample storage structure. This eliminates the need for separate verification equipment and sample rearrangement, allowing simultaneous storage and verification of multiple samples on a single holder.
Solution Approach 2:
The control area contains a control analyte that is identical to the target analyte in the biological sample. This copy allows the same staining procedure to be tested on the control analyte, providing a reliable verification of staining success without requiring separate verification experiments or sample rearrangement.
2Ease of operation
If control areas are integrated onto sample holders, then staining verification can be performed without rearranging samples, but the control area must be precisely positioned and deposited
Solution Approach 1:
The control area is pre-deposited on the sample holder at predetermined locations before the biological samples are mounted. This preliminary action ensures precise positioning and allows the control areas to be ready for immediate use during staining verification, eliminating the need for precise positioning during sample handling operations.
3Productivity
If multiple control areas are deposited on sample holders, then verification of multiple samples can be performed simultaneously, but the deposition process becomes more complex
Solution Approach 1:
The control area is deposited using a liquid solution that is applied via droplet deposition methods. This approach allows multiple control areas to be deposited in parallel or sequentially using fluid-based delivery systems, which are generally simpler and more scalable than solid-state deposition methods, thereby increasing verification throughput without proportionally increasing system complexity.
Applied Scientific Principles
This section explains which scientific principles are used to turn an abstract innovation direction into a practical engineering solution.
Function Achieved in This Case
This approach allows for efficient and flexible verification of staining procedures on existing collections of biological samples, ensuring successful binding of markers to target analytes and enabling accurate analysis without the need for rearrangement, thereby improving the handling and analysis efficiency.
Implementation Method 1
The control area may be generated by applying the flexible carrier with the control area onto the sample holder. The control area may be generated by depositing on the surface a solution comprising a control analyte and a polymerizing reagent.
Data Source
AI summary
A method for generating at least a first control area for a biological analysis includes depositing on a surface a solution comprising a first control analyte in order to generate the first control area. A system is configured to carry out the method. A method is also provided for analysing a biological sample including at least one first target analyte.