Copolymer Cell Preservation Composition for Room-Temperature Storage
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Solution Overview
Problem
Current cell preservation solutions require specific hypothermic preservation temperatures (2°C-8°C) and often use DMSO, which is physiologically toxic, leading to poor preservation efficiency, low cell survival rates, and difficulty in mass production, especially for autologous and allogeneic cell preparations.
Innovation Solution
A cell preservation solution composition comprising a copolymer of compound A (60.00wt%-99.5wt%) and compound B (0.05wt%-40.00wt%), which can be used at temperatures ranging from hypothermic to room temperature, excluding DMSO, and includes additives to reduce cell damage during preservation.
Engineering Contradictions & Design Principles
Engineering Contradiction Analysis
1Reliability
If DMSO is used as cryoprotectant, then cell protection during freezing is improved, but physiological toxicity increases causing hypertension, nausea, and vomiting
Solution Approach 1:
The patent removes DMSO from the preservation solution composition entirely, extracting the harmful substance while maintaining cell protection functionality through alternative components. The solution uses a combination of other cryoprotectants and additives to achieve protection without DMSO-induced toxicity.
Solution Approach 2:
The patent introduces intermediary substances such as human serum albumin, glucose, and other additives that mediate between the freezing process and cell protection, providing alternative mechanisms for cryoprotection that do not involve DMSO's toxic effects.
2Reliability
If hypothermic preservation at 2°C-8°C is used, then cell preservation is improved, but storage flexibility deteriorates requiring specific temperature control
Solution Approach 1:
The patent changes the compositional parameters of the preservation solution to include substances that provide protection across a broader temperature range. The specific formulation with human serum albumin, glucose, and other additives enables cells to be preserved not only at hypothermic temperatures but also at room temperature and during thawing processes.
3Reliability
If slow freezing method is used, then intracellular ice crystal formation is prevented, but preservation time and temperature control complexity increase
Solution Approach 1:
The patent employs a disposable preservation solution formulation that provides ice crystal prevention through its chemical composition rather than requiring complex controlled-rate freezing equipment. The solution itself acts as the protective mechanism, simplifying the preservation process to mixing cells with the solution and storing at simple temperature conditions.
4Reliability
If cell separation and washing processes are performed before preservation, then preservation purity is improved, but processing time and operational complexity increase
Solution Approach 1:
The preservation solution is designed with multi-functional properties: it provides cryoprotection, maintains cell viability, and can be directly applied to cell suspensions without requiring prior separation or washing steps. The solution's composition allows it to function effectively whether cells are freshly isolated or from various sources, reducing preprocessing requirements.
Applied Scientific Principles
This section explains which scientific principles are used to turn an abstract innovation direction into a practical engineering solution.
Function Achieved in This Case
The solution extends storage time and improves cell survival rates, allowing cells to be preserved and thawed without loss of functionality, even at room temperature, and supports mass production without the need for low-temperature storage.
Implementation Method 1
cryoprotectant (CPA) are used to freeze cells. It is used to protect the vitality of cells and tissues
Implementation Method 2
the formation of intracellular ice crystals and the imbalance of osmotic pressure may damage cells
Implementation Method 3
If the temperature is rapidly cooled, a vitrified state can be induced in most liquids
Implementation Method 4
the consistency of the sample reaches a certain level, and it becomes amorphous and fixed
Data Source
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AI summary
A cell preservation solution composition, excluding dimethyl sulfoxide (DMSO), includes a copolymer of compound A and compound B. In a composition of 100 wt% of the cell preservation solution composition, the compound A ranges from 60.00wt% to 99.95wt%, and compound B ranges from 0.05 wt% to 40.00 wt%.