Water-Insoluble Copolymer Coating for Cell Culture Vessels
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Solution Overview
Problem
Current coating materials fail to effectively suppress adhesion of biological substances on medical devices and cell culture vessels while remaining insoluble in phosphate buffered saline.
Innovation Solution
A water-insoluble copolymer coating film, composed of specific repeating units with a defined molar ratio and viscosity-average polymerization degree, is applied to prevent biological substance adhesion and maintain stability in phosphate buffered saline.
Engineering Contradictions & Design Principles
Engineering Contradiction Analysis
1Reliability
If coating materials are applied to suppress adhesion of biological substances, then adhesion suppression ability is improved, but dissolution in phosphate buffered saline occurs
Solution Approach 1:
The patent applies composite materials by creating a copolymer that combines two different repeating units: repeating unit (A) with hydrophobic characteristics and repeating unit (B) with hydrophilic characteristics. This composite structure at the molecular level allows the coating film to exhibit both adhesion suppression ability and stability in phosphate buffered saline, resolving the contradiction between these two properties.
Solution Approach 2:
The patent applies parameter changes by precisely controlling the molar ratio of repeating units (A) and (B) in the copolymer within the range of 70:30 to 95:5. Additionally, the viscosity-average polymerization degree is controlled within 100 to 3,000. These parameter optimizations enable the coating film to achieve both adhesion suppression and stability in phosphate buffered saline without dissolution.
2Stability of the object's composition
If crosslinking is performed to improve stability, then dissolution resistance is improved, but manufacturing complexity increases
Solution Approach 1:
The patent applies the taking out principle by extracting the crosslinking step from the manufacturing process. Instead of requiring crosslinking to achieve stability and dissolution resistance, the invention uses a specifically designed copolymer composition that inherently provides these properties through its molecular structure, thereby simplifying the manufacturing process.
Data Source
AI summary
Provided is a coating film which is obtained by applying a composition for forming a coating film, the composition containing a copolymer, and which is used to suppress adhesion of a biological substance, in which the copolymer is water-insoluble, the copolymer contains a repeating unit (A) represented by the following Formula (A) and a repeating unit (B) represented by the following Formula (B), and a molar ratio (A:B) of the repeating unit (A) to the repeating unit (B) in the copolymer is 89:11 to 50:50.(In the formula, R1 to R3 each independently represent a hydrogen atom or an alkyl group having 1 to 5 carbon atoms, and X1 and X2 each independently represent a single bond, an ester bond, an ether bond, an amide bond, or an alkylene group having 1 to 5 carbon atoms which may be interrupted by an oxygen atom.)