Copolymer Solid Phase Carrier for Low-Background Ligand Binding
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Solution Overview
Problem
Existing solid phase carriers achieve high non-specific adsorption inhibitory effect but have a low ligand binding amount, limiting their sensitivity in fluorescence detection methods.
Innovation Solution
A solid phase carrier with a copolymer having a structural unit where a hydrophilic group and a bioactive substance-reactive group are bonded to an acidic amino acid-derived group through a linking group, enhancing both non-specific adsorption inhibition and ligand binding capacity.
Engineering Contradictions & Design Principles
Engineering Contradiction Analysis
1Object-affected harmful factors
If a solid phase carrier uses a polymer with high non-specific adsorption inhibitory effect, then non-specific adsorption is inhibited, but the ligand binding amount becomes small
Solution Approach 1:
The patent applies local quality by creating a copolymer structure where different segments of the polymer chain have different functional properties: one segment provides non-specific adsorption inhibition through hydrophilic groups, while another segment provides ligand binding through bioactive substance-reactive groups. This allows the carrier surface to simultaneously exhibit both non-specific adsorption resistance and high ligand binding capacity, resolving the contradiction between these two opposing requirements.
Solution Approach 2:
The patent uses composite materials by constructing a copolymer that combines multiple functional units: hydrophilic groups (providing non-specific adsorption inhibition), bioactive substance-reactive groups (providing ligand binding), and acidic amino acid-derived groups (providing both non-specific adsorption inhibition and ligand binding). This composite polymer structure enables the carrier to achieve both high non-specific adsorption inhibitory effect and high ligand binding amount simultaneously.
2Measurement precision
If a solid phase carrier has high non-specific adsorption inhibitory effect, then measurement precision is improved, but the sensitivity is limited due to low ligand binding amount
Solution Approach 1:
The patent applies local quality by creating a copolymer structure where different segments of the polymer chain have different functional properties: one segment provides non-specific adsorption inhibition through hydrophilic groups, while another segment provides ligand binding through bioactive substance-reactive groups. This allows the carrier surface to simultaneously exhibit both non-specific adsorption resistance and high ligand binding capacity, resolving the contradiction between these two opposing requirements.
Solution Approach 2:
The patent uses composite materials by constructing a copolymer that combines multiple functional units: hydrophilic groups (providing non-specific adsorption inhibition), bioactive substance-reactive groups (providing ligand binding), and acidic amino acid-derived groups (providing both non-specific adsorption inhibition and ligand binding). This composite polymer structure enables the carrier to achieve both high non-specific adsorption inhibitory effect and high ligand binding amount simultaneously.
Applied Scientific Principles
This section explains which scientific principles are used to turn an abstract innovation direction into a practical engineering solution.
Function Achieved in This Case
The carrier achieves a high signal/noise ratio for sensitive detection of measurement target substances by simultaneously inhibiting non-specific adsorption and increasing ligand binding, allowing for accurate analysis with high sensitivity.
Implementation Method 1
a copolymer having a structural unit in which a hydrophilic group and a bioactive substance-reactive group are bonded to an acidic amino acid-derived group through a present linking group
Implementation Method 2
a copolymer having a structural unit in which a hydrophilic group and a bioactive substance-reactive group are bonded to an acidic amino acid-derived group through a present linking group
Implementation Method 3
a sensor chip having a metal film provided in a predetermined area on a transparent support is prepared, and excitation light is caused to incident on the interface between the support and the metal film from one surface of the support opposite to the other surface thereof provided with the metal film at a predetermined angle equal to or larger than the angle of total reflection. Due to the irradiation with the excitation light, surface plasmon occurs on the metal film, and the occurrence of the surface plasmon enhances the electric field.
Data Source
AI summary
An object of the present invention is to provide a solid phase carrier for carrying a bioactive substance, which achieves both the high non-specific adsorption inhibitory effect and high ligand binding amount. Another object of the present invention is to provide a kit for measuring a measurement target substance in a biosample, which includes the solid phase carrier. According to an embodiment of the present invention, a solid phase carrier for carrying a bioactive substance is provided which includes a copolymer present on a surface of the solid phase. The copolymer has a structural unit in which a hydrophilic group and a bioactive substance-reactive group are bonded to an acidic amino acid-derived group through a predetermined linking group.