Copper Coordination Polymer Viral RNA Detection
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Solution Overview
Problem
Current methods for detecting viral RNA, such as Dengue and Zika viruses, face challenges with poor water stability and limited sensitivity, especially in simultaneous and synchronous detection, which is crucial for distinguishing between similar diseases.
Innovation Solution
A crystalline copper-based coordination polymer is synthesized using specific pyridyl ligands, allowing for the formation of a 3D structure that interacts with fluorophore-labeled oligonucleotide probes to quench fluorescence, enabling sensitive and specific detection of multiple viral RNA sequences.
Engineering Contradictions & Design Principles
Engineering Contradiction Analysis
1Measurement precision
If metal-organic frameworks (MOFs) are used as quenching platforms for fluorophore-labeled nucleic acid, then sensitivity for target nucleic acid detection is enhanced, but water stability deteriorates
Solution Approach 1:
The patent changes the chemical composition parameters of the MOF by selecting specific copper-based coordination polymers with defined ligands (carboxylate pyridyl ligands and polypyridyl ligands) to achieve both high sensitivity and water stability. The copper-based coordination polymer [Cu(Dcbcp)(bpe)]n was synthesized with specific stoichiometric ratios and purified through recrystallization to obtain crystals with optimized properties for both sensing performance and aqueous stability.
Solution Approach 2:
The patent creates a composite sensing system combining the copper-based coordination polymer with fluorophore-labeled oligonucleotide probes. The coordination polymer serves as the quenching platform while the fluorescent probes provide detection capability, forming a synergistic composite material that achieves both high sensitivity and water stability for viral RNA detection.
2Measurement precision
If separate detection methods are used for different viral nucleic acids, then detection specificity is maintained, but analysis time increases
Solution Approach 1:
The copper-based coordination polymer serves as a universal sensing platform that can simultaneously detect multiple viral RNA sequences (Dengue and Zika viruses) using different fluorophore-labeled oligonucleotide probes. The platform's ability to bind and quench fluorescence from multiple probe types enables multiplexed detection of different pathogens in a single assay, maintaining specificity while reducing analysis time.
Solution Approach 2:
The patent merges the detection of multiple viral nucleic acids into a single simultaneous assay using the copper-based coordination polymer platform. By combining Dengue and Zika virus detection in one experiment with shared reagents and analysis流程, the system achieves time savings while maintaining the ability to distinguish between different viral sequences through specific probe-target hybridization.
3Speed
If fluorescent biosensing is used for viral detection, then response time is reduced, but background fluorescence interference increases
Solution Approach 1:
The patent converts the harmful background fluorescence into a beneficial detection signal by using the copper-based coordination polymer as a quenching platform. The polymer's ability to quench fluorescence from bound probes creates a signal change that can be detected, transforming the potential interference into the basis for sensitive detection while maintaining rapid response time.
Applied Scientific Principles
This section explains which scientific principles are used to turn an abstract innovation direction into a practical engineering solution.
Function Achieved in This Case
The method achieves high selectivity and sensitivity for simultaneous detection of Dengue and Zika virus RNA, with improved detection limits and reduced analysis time, demonstrating potential for early diagnosis of viral infectious diseases.
Implementation Method 1
interacts with fluorophore-labeled oligonucleotide probes to quench fluorescence
Data Source
AI summary
A method of preparing a crystalline copper-based coordination polymer comprises preparing a mixture of copper ions and a first quaternized carboxylate pyridyl ligand; adding a second polypyridyl ligand; and forming crystals of a copper-based coordination polymer therefrom. The crystalline copper-based coordination polymer is suitable for providing a sensing platform for detecting the presence of one or more target nucleic acid sequences such as viral RNA, in particular Dengue virus and/or Zika virus RNA with high selectivity and high specificity. A method of detecting at least a first target nucleic acid sequence, in particular from a viral RNA, in particular it is Flavivirus RNA, in a sample is also provided. Further provided is a kit including the crystalline copper-based coordination polymer and an oligonucleotide probe.