CoQ10 Fermentation Control via CO2 and Lactate Regulation
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Solution Overview
Problem
The CoQ10 fermentation production process lacks comprehensive control, leading to fluctuations in yield and quality due to biofilm formation affecting material transfer between microbes and the fermentation medium, resulting in inconsistent production.
Innovation Solution
A controlled CoQ10 fermentation process is implemented by maintaining specific concentrations of carbon dioxide and lactate in the microbial culture over varying time periods, using adjustments in rotor speed, tank pressure, and air supply rate to optimize microbial growth and biofilm inhibition, with the addition of D-amino acids when necessary to prevent biofilm formation.
Engineering Contradictions & Design Principles
Engineering Contradiction Analysis
1Manufacturing precision
If comprehensive control is implemented in the fermentation production process, then yield and quality consistency is improved, but process complexity increases
Solution Approach 1:
The patent implements feedback control by monitoring carbon dioxide concentration and lactate levels in real-time, and adjusting rotor speed, tank pressure, and air supply rate accordingly. This closed-loop control system ensures consistent CoQ10 production by automatically responding to process parameter changes, resolving the contradiction between achieving consistent yield and avoiding complex control mechanisms.
Solution Approach 2:
The patent systematically varies key process parameters (rotor speed, tank pressure, air supply rate) at different fermentation stages to optimize CoQ10 production. By dynamically adjusting these parameters based on time and measured concentrations, the process achieves consistent results without requiring overly complex control systems.
2Productivity
If biofilm formation is allowed to occur naturally, then microbial growth is promoted, but material transfer efficiency deteriorates
Solution Approach 1:
The patent applies preliminary anti-action by adding D-amino acids to the fermentation medium to prevent biofilm formation before it adversely affects material transfer. This proactive approach maintains microbial growth while preventing the harmful effects of biofilm accumulation, thus preserving material transfer efficiency.
Solution Approach 2:
The patent adjusts tank pressure and air supply rate as process parameters to control biofilm formation. By optimizing these parameters, the process promotes beneficial microbial growth while preventing excessive biofilm development that would hinder material transfer.
3Object-generated harmful factors
If rotor speed is increased to improve material transfer, then oxygen and carbon source transfer is enhanced, but energy consumption increases
Solution Approach 1:
The patent applies dynamics by varying rotor speed according to the fermentation stage and real-time process conditions. The rotor speed is adjusted dynamically rather than maintained at a constant high level, optimizing material transfer efficiency while minimizing energy consumption during different phases of CoQ10 production.
Solution Approach 2:
The patent systematically changes rotor speed as a process parameter based on time intervals and measured fermentation parameters. This controlled adjustment ensures adequate material transfer without excessive energy consumption, achieving an optimal balance between productivity and energy use.
Applied Scientific Principles
This section explains which scientific principles are used to turn an abstract innovation direction into a practical engineering solution.
Function Achieved in This Case
This approach stabilizes CoQ10 production by maintaining optimal carbon dioxide and lactate levels, improving material transfer efficiency and preventing biofilm formation, thereby ensuring consistent yield and quality across batches.
Implementation Method 1
CoQ10 may be produced through a fermentation production process using a microorganism strain, such as Rhodobacter sphaeroides
Data Source
AI summary
Controlled coenzyme Q10 (CoQ10) fermentation production processes and methods for controlling the CoQ10 fermentation production processes are provided in the present disclosure. The processes may include growing a microbial culture of bacteria by providing a carbon source and an oxygen source for a predetermined period of time, thereby producing CoQ10-containing bacteria, carbon dioxide, and lactate in the bacterial culture. During various stages of the production process, the concentration of carbon dioxide may be maintained at predetermined levels, respectively. Alternatively or additionally, during various stages of the production process, the concentration of lactate may be maintained at predetermined levels, respectively.