Umbilical Cord Extract Preservation Through Cold Buffered Filtration

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Solution Overview

Problem

Existing methods for obtaining umbilical cord extracts do not reliably preserve the regenerative properties of the cells, leading to inconsistent and ineffective results in medical and cosmetic applications.

Innovation Solution

A method involving the preservation of umbilical cords in a buffered solution, followed by washing, fractionating and homogenizing in buffered cell culture medium, centrifuging, and filtering through specific pore sizes to maintain the extract's structural stability and biological properties at low temperatures.

Engineering Contradictions & Design Principles

VSEngineering Contradiction Analysis

1Reliability

If conventional methods are used to obtain umbilical cord extracts, then the extraction process is simple, but the regenerative properties of the extract are not preserved and results are inconsistent

Engineering Contradiction:

Improveregenerative properties preservationVSAvoidextraction process complexity

Core Design Contradiction:

ReliabilityVSDevice complexity

Solution Approach 1:

The extraction process is divided into multiple sequential steps: initial homogenization, first centrifugation, first filtration (0.45 μm), second centrifugation, and second filtration (0.22 μm). Each step isolates and purifies specific components, ensuring consistent regenerative properties while making the complex process manageable through systematic segmentation

Inventive Principle:

Principle #1Segmentation

Solution Approach 2:

Umbilical cords are collected and stored in buffered solution at controlled temperatures (2-8°C) before processing. This preliminary preservation maintains cellular integrity and regenerative properties from the moment of collection, ensuring consistent results throughout the extraction process

Inventive Principle:

Principle #10Preliminary action

2Manufacturing precision

If multiple filtration steps are performed, then the purity of the extract is improved, but the processing time increases

Engineering Contradiction:

Improveextract purityVSAvoidprocessing time

Core Design Contradiction:

Manufacturing precisionVSLoss of time

Solution Approach 1:

The extraction process employs continuous centrifugation and filtration steps without interruption. Crude extract is immediately centrifuged and filtered, then the filtrate undergoes sequential centrifugation and filtration without delay, maintaining continuous purification action that achieves high purity while minimizing idle time

Inventive Principle:

Principle #20Continuity of useful action

Solution Approach 2:

Buffered solutions serve as intermediaries throughout the process, maintaining pH and ionic balance during homogenization, centrifugation, and filtration. These intermediary buffers protect regenerative components while enabling efficient processing through standardized conditions

Inventive Principle:

Principle #24Intermediary (Mediator)

3Manufacturing precision

If centrifugation is performed at high speed, then the separation efficiency is improved, but the cellular structures may be damaged

Engineering Contradiction:

Improveseparation efficiencyVSAvoidcellular structure integrity

Core Design Contradiction:

Manufacturing precisionVSStability of the object's composition

Solution Approach 1:

The protocol employs two centrifugation steps with different speeds: initial centrifugation at moderate speed to remove large debris, followed by a second centrifugation at higher speed to separate finer particles. This partial action approach achieves thorough separation while avoiding excessive force that would damage cellular structures

Inventive Principle:

Principle #16Partial or excessive action

Solution Approach 2:

Different centrifugation speeds are applied at different stages of the process. The first centrifugation uses lower g-force for bulk separation, while the second uses higher g-force for fine purification. This localized adjustment of centrifugal force optimizes separation efficiency at each stage without compromising overall cellular integrity

Inventive Principle:

Principle #3Local quality

Applied Scientific Principles

This section explains which scientific principles are used to turn an abstract innovation direction into a practical engineering solution.

Function Achieved in This Case

The method ensures the umbilical cord extract retains its regenerative capacity, enhancing cell proliferation and migration, as demonstrated by increased cell metabolic activity and wound healing capabilities.

Implementation Method 1

reserving between one and five umbilical cords after birth in a physiological or buffered solution

Methodology Applied

Scientific EffectBuffering:

Implementation Method 2

centrifuge the mixture obtained in b—

Methodology Applied

Scientific EffectCentrifugal separation: Centrifugal Separation

Implementation Method 3

filter the crude liquid fraction obtained with a clarification filter between 1 μm to 100 μm, followed by a second filtration by pore size for microfiltration between 0.1 μm to 1 μm

Methodology Applied

Scientific EffectPhysical filtration: Filter (physical)

Implementation Method 4

all steps of the method are carried out at a temperature between 1 and 10° C.

Methodology Applied

Scientific EffectThermal preservation: Cooling

Data Source

PatentUS20260076998A1Method for obtaining an umbilical cord extract

Publication Date: 2026.03.19 UNIV SAN FRANCISCO DE QUITO USFQ

AI summary

The present invention relates to a method for obtaining an umbilical cord extract that can be used for tissue regeneration or repair, for medical or cosmetic uses, as a preparation base for liquid, semisolid or solid grafts or dressings, binders or polymerising agents, or in the subsequent purification of the regenerative components thereof.