Umbilical Cord Adherent Stem Cell Enrichment for Homogeneous Isolation

Resolve Bottlenecks,
Find Innovative SolutionsGenerate Solutions

Solution Overview

Problem

Existing methods for obtaining stem cells from bone marrow and adipose tissue are invasive, and cord blood-derived stem cells have low content, while umbilical cord-derived cells are non-invasive but require optimization for homogeneous cell populations and enhanced therapeutic potential.

Innovation Solution

A method involving adherent-culturing umbilical cord tissue, using dissociation enzymes, and subculturing with fibroblast growth factor-4 (FGF-4) and heparin to isolate and enhance umbilical cord-derived adherent stem cells, which express specific markers and have high differentiation potential.

Engineering Contradictions & Design Principles

VSEngineering Contradiction Analysis

1Reliability

If stem cells are collected from bone marrow or adipose tissue, then stem cells can be obtained with certain differentiation and proliferation ability, but the collection method is invasive

Engineering Contradiction:

Improvedifferentiation and proliferation abilityVSAvoidinvasive collection method

Core Design Contradiction:

ReliabilityVSObject-affected harmful factors

Solution Approach 1:

The patent extracts stem cells from umbilical cord tissue, which is discarded after delivery, rather than collecting from bone marrow or adipose tissue. This extraction approach obtains stem cells with high differentiation and proliferation ability while avoiding invasive procedures, as the umbilical cord is already separated from the body

Inventive Principle:

Principle #2Taking out (Extraction)

Solution Approach 2:

The patent performs preliminary isolation and enrichment of stem cells from umbilical cord tissue before transplantation. By pre-processing the umbilical cord tissue to isolate stem cells and culture them under optimized conditions, the patent ensures high cell quality and functionality while maintaining the non-invasive advantage of umbilical cord as the source

Inventive Principle:

Principle #10Preliminary action

2Ease of operation

If stem cells are isolated from cord blood, then collection is non-invasive and easy, but the content of stem cells is low

Engineering Contradiction:

Improvecollection easeVSAvoidstem cell content

Core Design Contradiction:

Ease of operationVSQuantity of substance

Solution Approach 1:

The patent uses umbilical cord tissue as an intermediary source between cord blood and bone marrow/adipose tissue. The umbilical cord provides easier collection than bone marrow while yielding higher stem cell content than cord blood. The tissue is processed through enzymatic digestion and culture to enrich stem cells, achieving both ease of collection and high cell content

Inventive Principle:

Principle #24Intermediary (Mediator)

Solution Approach 2:

The patent changes the physical state and processing parameters of umbilical cord tissue by using enzymatic digestion, mechanical dissociation, and optimized culture conditions. These parameter changes transform the raw tissue into a source of highly concentrated stem cells, increasing the effective stem cell content from the easily collected umbilical cord

Inventive Principle:

Principle #35Parameter changes

3Reliability

If umbilical cord-derived cells are used, then non-invasive collection and high differentiation ability are achieved, but homogeneous cell populations need to be isolated

Engineering Contradiction:

Improvedifferentiation abilityVSAvoidcell population homogeneity

Core Design Contradiction:

ReliabilityVSManufacturing precision

Solution Approach 1:

The patent segments the heterogeneous umbilical cord tissue into distinct cell populations through enzymatic digestion and mechanical dissociation. By breaking down the tissue into individual cells and then culture-selecting for adherent stem cells with specific markers, the patent isolates homogeneous populations from the mixed tissue, maintaining high differentiation ability while achieving cellular uniformity

Inventive Principle:

Principle #1Segmentation

Solution Approach 2:

The patent employs feedback mechanisms through marker-based selection and phenotypic characterization during the isolation and culture process. By monitoring cell surface markers and differentiation markers, the patent adjusts culture conditions and selection criteria to enrich for homogeneous stem cell populations with the desired differentiation potential

Inventive Principle:

Principle #23Feedback

Applied Scientific Principles

This section explains which scientific principles are used to turn an abstract innovation direction into a practical engineering solution.

Function Achieved in This Case

The enhanced umbilical cord-derived adherent stem cells exhibit high expression of specific genes, maintain fibroblast morphology, and differentiate into various cell types, offering therapeutic benefits for inflammatory, ischemic, and neurodegenerative diseases.

Implementation Method 1

isolating enhanced umbilical cord-derived adherent stem cells by contacting the cultured umbilical cord with a dissociation enzyme

Methodology Applied

Scientific EffectEnzyme: Enzyme

Data Source

PatentUS12478646B2Umbilical cord-derived adherent stem cells, preparation method therefor, and use thereof

Publication Date: 2025.11.25 CHABIO & DIOSTECH CO LTD

AI summary

Disclosed are enhanced umbilical cord-derived adhesive stem cells, a preparation method therefor, and a use thereof. The enhanced umbilical cord-derived adhesive stem cells have an anti-inflammatory effect, a blood vessel regeneration effect, or a nerve regeneration effect, thereby being usable in a pharmaceutical composition or a cell therapeutic agent for treating or preventing various diseases.