Core-Shell Chromatography Carrier with Porous Siloxane Shell
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Solution Overview
Problem
Conventional chromatography separating agents using wholly porous silica gel as carriers face limitations in efficiently separating target substances due to their structure and ligand binding methods, which can affect separation efficiency and specificity.
Innovation Solution
A core-shell particle with a non-porous core and a porous polyalkoxysiloxane shell is used as the carrier, with ligands such as optically active polymers, proteins, and optically inactive polyesters chemically bonded to the surface, enhancing the specific surface area and separation capabilities.
Engineering Contradictions & Design Principles
Engineering Contradiction Analysis
1Reliability
If wholly porous silica gel is used as a carrier, then ligands can be fixed to the carrier, but the separation efficiency and specificity are limited
Solution Approach 1:
The carrier is divided into two distinct parts: a non-porous core and a porous shell. This segmentation allows the non-porous core to provide structural stability while the porous shell provides the necessary surface area for ligand fixation, thereby improving separation efficiency without compromising structural integrity
Solution Approach 2:
The carrier is constructed as a composite structure combining a non-porous core material with a porous shell material. This composite design enables the carrier to simultaneously exhibit mechanical strength from the non-porous core and high surface area for ligand binding from the porous shell, resolving the contradiction between reliability and device complexity
2Reliability
If ligands are fixed to the carrier by chemical bonding, then separation specificity is improved, but the specific surface area for ligand loading is reduced
Solution Approach 1:
The invention transitions from a fully porous three-dimensional structure to a core-shell structure with a distinct surface layer. This dimensional reorganization concentrates the ligand fixation functionality on the outer porous shell surface, maximizing the specific surface area available for chemical bonding while maintaining high separation specificity through selective ligand placement
Applied Scientific Principles
This section explains which scientific principles are used to turn an abstract innovation direction into a practical engineering solution.
Function Achieved in This Case
The core-shell particle design increases the specific surface area and ligand loading, leading to improved separation efficiency and specificity of target substances, as demonstrated by enhanced retention coefficients and separation coefficients in chromatography applications.
Implementation Method 1
a porous shell covering the outer surface of the core, the shell having a pore diameter of 9 nm or more
Implementation Method 2
a ligand fixed on the surface of the carrier by chemical bonding
Data Source
AI summary
Provided is a separating agent comprising a carrier and a ligand fixed on a surface of a carrier by chemical bonding, in which the carrier is a core-shell particle formed of a nonporous core and a porous shell, the shell having a pore diameter of 9 nm or more and formed of a hydrolysate of polyalkoxysiloxane, and the ligand is an optically active polymer, optically inactive polyester, protein, nucleic acid, or optically active organic compound with a molecular weight of 50 to 1000.