Core-Shell Microparticles Enhance HGF Gene Expression
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Solution Overview
Problem
Current methods fail to effectively increase the expression of growth factor genes, such as HGF and IGF1, in vivo, especially when administered without ultrasound irradiation.
Innovation Solution
The use of core-shell structured microparticles with a halogenated hydrocarbon or halogenated sulfur core and a lipid outer shell, which are administered in vivo along with the HGF or IGF1 gene, significantly enhance gene expression.
Engineering Contradictions & Design Principles
Engineering Contradiction Analysis
1Reliability
If core-shell structured microparticles are administered along with growth factor genes, then gene expression is increased by at least 30%, but the method is only effective for specific growth factor genes (HGF and IGF1) and not for other growth factor genes
Solution Approach 1:
The patent applies local quality by creating microparticles with specific local characteristics - a halogenated hydrocarbon core providing structural stability and a lipid shell providing biocompatibility and cellular uptake facilitation. This localized functional differentiation within the microparticle structure enables selective enhancement of HGF and IGF1 gene expression while maintaining the ability to be adapted for other genes through formulation adjustments
2Quantity of substance
If small amounts of growth factor genes are administered, then therapeutic effects are achieved through enhanced expression, but the effectiveness is limited to specific growth factor genes
Solution Approach 1:
The patent utilizes parameter changes by modifying the physical and chemical properties of the microparticle system - specifically the core shell structure with halogenated hydrocarbon and lipid components. These parameter modifications create an enhanced expression system that works effectively with minimal gene amounts for HGF and IGF1, while the parameters can be adjusted to extend effectiveness to other growth factor genes
3Ease of operation
If conventional gene administration methods are used, then the process is simple, but gene expression is not effectively increased
Solution Approach 1:
The patent introduces core-shell structured microparticles as an intermediary carrier between the growth factor gene and the target cells. These microparticles mediate the delivery process, protecting the gene and facilitating its entry into cells, thereby significantly enhancing expression while maintaining a relatively simple administration process that can be extended to multiple gene types
Data Source
AI summary
The present disclosure relates to a composition for increasing the expression of a growth factor gene, which contains core-shell structured microparticles as an active ingredient. When administered in vivo along with a growth factor gene, the composition for increasing the expression of a growth factor gene of the present disclosure can increase the expression of the co-administered gene by at least 30%. Especially, when administered along with at least one gene selected from a human hepatocyte growth factor (HGF) gene, an isoform gene of the human hepatocyte growth factor and a variant gene thereof, or at least one gene selected from a human insulin-like growth factor 1 (IGF1) gene, an isoform gene of the human insulin-like growth factor 1 and a variant gene thereof, which are appropriate for the present disclosure, the composition can increase the expression of the gene by at least 30%. When administered along with a gene therapeutic agent, the composition can achieve a therapeutic effect even with a very small amount of a gene, and thus is useful.