Hybrid Corn CH101514 Cytoplasmic Male Sterility Breeding

Resolve Bottlenecks,
Find Innovative Solutions
Generate Solutions

Solution Overview

Problem

Current corn breeding techniques face challenges in developing hybrid varieties that combine desirable traits such as high yield, disease resistance, and uniformity, while maintaining genetic stability and avoiding self-pollination, which limits the efficiency of trait introduction and hybrid seed production.

Innovation Solution

The development of the hybrid corn variety CH101514, which incorporates genetic modifications and cytoplasmic male sterility traits, along with advanced breeding methods like backcrossing and genetic transformation, to introduce traits like herbicide resistance, disease resistance, and improved agronomic characteristics, while preventing self-pollination through male sterility mechanisms.

Engineering Contradictions & Design Principles

VSEngineering Contradiction Analysis

1Productivity

If self-pollination is allowed in corn breeding, then genetic stability is maintained, but hybrid seed production efficiency decreases due to uncontrollable self-pollination

Engineering Contradiction:
Improvehybrid seed production efficiencyVSAvoidgenetic stability
Core Design Contradiction:
ProductivityVSStability of the object's composition

Solution Approach 1:

The invention extracts and removes the male reproductive function from the female parent plant through cytoplasmic male sterility (CMS). The female parent plant lacks functional pollen due to CMS, completely eliminating self-pollination capability while maintaining genetic stability of the female parent. This allows efficient hybrid seed production without the harmful effect of self-pollination.

Inventive Principle:
Principle #2Taking out (Extraction)

Solution Approach 2:

The invention introduces a male parent plant as an intermediary that provides pollen for cross-pollination. The male parent plant is specifically selected or engineered to carry desirable traits, ensuring that cross-pollination introduces beneficial genetic variation while the female parent's CMS prevents unwanted self-pollination. This intermediary mechanism resolves the contradiction between maintaining genetic stability and enabling controlled hybridization.

Inventive Principle:
Principle #24Intermediary (Mediator)

2Productivity

If traditional breeding methods are used, then trait introduction is possible, but the process is time-consuming and less efficient

Engineering Contradiction:
Improvebreeding efficiencyVSAvoidbreeding time
Core Design Contradiction:
ProductivityVSLoss of time

Solution Approach 1:

The invention performs preliminary action by developing and maintaining inbred lines with cytoplasmic male sterility before hybrid seed production. The female parent line is pre-engineered or pre-selected to have CMS, and the male parent line is pre-selected for desirable traits. This preliminary preparation eliminates the need for time-consuming emasculation and selection processes during hybrid seed production, significantly improving breeding efficiency and reducing time loss.

Inventive Principle:
Principle #10Preliminary action

Solution Approach 2:

The cytoplasmic male sterility system provides self-service by automatically preventing self-pollination in the female parent without requiring human intervention for emasculation or manual pollination control. The CMS trait inherently ensures that the female parent can only receive pollen from the male parent, streamlining the breeding process and reducing labor-intensive operations while accelerating hybrid seed production.

Inventive Principle:
Principle #25Self-service

3Productivity

If male sterility is not used, then self-pollination control is difficult, but hybrid seed production becomes less efficient

Engineering Contradiction:
Improvehybrid seed production efficiencyVSAvoidpollination control mechanism
Core Design Contradiction:
ProductivityVSDevice complexity

Solution Approach 1:

The cytoplasmic male sterility system enables the female parent plant to self-regulate and prevent self-pollination automatically through its genetic makeup. The CMS trait in the female parent's cytoplasm ensures that its pollen is non-functional, so self-pollination cannot occur regardless of environmental conditions or manual intervention. This self-service mechanism simplifies pollination control and dramatically improves hybrid seed production efficiency without adding operational complexity.

Inventive Principle:
Principle #25Self-service

Solution Approach 2:

The invention changes the fundamental parameter of pollen functionality in the female parent from functional to non-functional through cytoplasmic male sterility. This parameter change transforms the pollination control mechanism from requiring external physical or chemical intervention to an inherent genetic property. The CMS parameter ensures complete prevention of self-pollination while allowing efficient cross-pollination, resolving the contradiction between production efficiency and control complexity.

Inventive Principle:
Principle #35Parameter changes

Data Source

PatentUS20200396941A1Plants and seeds of hybrid corn variety ch101514
Publication Date: 2020.12.24 MONSANTO TECHNOLOGY LLC

AI summary

According to the invention, there is provided seed and plants of the hybrid corn variety designated CH101514. The invention thus relates to the plants, seeds and tissue cultures of the variety CH101514, and to methods for producing a corn plant produced by crossing a corn plant of variety CH101514 with itself or with another corn plant, such as a plant of another variety. The invention further relates to genetic complements of plants of variety CH101514.