Hybrid Corn CH267703 Breeding via Cytoplasmic Male Sterility

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Solution Overview

Problem

Current corn breeding techniques face challenges in developing hybrid varieties that combine desirable traits such as high yield, disease resistance, and uniformity, while maintaining genetic stability and avoiding self-pollination, which limits the introduction of new genetic traits.

Innovation Solution

The development of the hybrid corn variety CH267703, which incorporates cytoplasmic or nuclear factors for male sterility, genetic modifications for herbicide resistance, disease resistance, and altered metabolic traits, along with a method for crossing parent plants to produce seeds with specific characteristics, utilizing techniques like backcrossing and genetic transformation to introduce desired loci.

Engineering Contradictions & Design Principles

VSEngineering Contradiction Analysis

1Adaptability or versatility

If traditional self-pollination breeding methods are used, then genetic stability is maintained, but the introduction of new genetic traits is limited

Engineering Contradiction:
Improveintroduction of new genetic traitsVSAvoidgenetic stability
Core Design Contradiction:
Adaptability or versatilityVSStability of the object's composition

Solution Approach 1:

The patent uses cytoplasmic male sterility (CMS) as an intermediary mechanism to prevent self-pollination and force cross-pollination. The CMS trait acts as a mediator that eliminates pollen production in female parent plants, ensuring that only cross-pollinated seeds are produced. This resolves the contradiction by enabling controlled introduction of new genetic traits through cross-breeding while maintaining genetic stability through the use of specific cytoplasmic and nuclear gene combinations.

Inventive Principle:
Principle #24Intermediary (Mediator)

Solution Approach 2:

The patent applies preliminary genetic modification to parent plants before crossing, specifically introducing cytoplasmic male sterility traits and desired genetic loci (such as herbicide resistance, disease resistance, or metabolic traits) into the parental lines. This preliminary action ensures that when crossing occurs, the offspring automatically inherit both the sterility mechanism and the desired traits, eliminating the need for subsequent self-pollination breeding steps.

Inventive Principle:
Principle #10Preliminary action

2Adaptability or versatility

If cross-pollination is used to introduce new traits, then genetic diversity increases, but self-pollination control becomes difficult

Engineering Contradiction:
Improvegenetic diversityVSAvoidself-pollination control mechanism
Core Design Contradiction:
Adaptability or versatilityVSDevice complexity

Solution Approach 1:

The patent employs cytoplasmic male sterility (CMS) as a biological intermediary that automatically controls pollination without requiring mechanical or manual intervention. The CMS cytoplasm interacts with nuclear restorer genes to create a self-regulating system: female parents with CMS cytoplasm and no restorer genes cannot produce pollen, while male parents with restorer genes produce pollen that can fertilize the female parents. This resolves the complexity of self-pollination control by using a genetic switch rather than mechanical emasculation or isolation methods.

Inventive Principle:
Principle #24Intermediary (Mediator)

Solution Approach 2:

The patent creates a self-service pollination control system where the plants themselves regulate their own pollination behavior through their genetic composition. Plants with CMS cytoplasm automatically become female-only (sterile in pollen production), while plants with restorer genes automatically become male parents. This self-service mechanism eliminates the need for external control devices or manual intervention, reducing system complexity while ensuring complete cross-pollination control.

Inventive Principle:
Principle #25Self-service

3Adaptability or versatility

If hybrid varieties are developed through multiple breeding steps, then desirable traits are combined, but breeding time and complexity increase

Engineering Contradiction:
Improvecombination of desirable traitsVSAvoidbreeding time
Core Design Contradiction:
Adaptability or versatilityVSLoss of time

Solution Approach 1:

The patent applies preliminary action by pre-establishing inbred lines with specific desirable traits and cytoplasmic male sterility characteristics before the hybridization step. Parental lines are developed in advance with predetermined traits (such as herbicide resistance, disease resistance, or improved metabolism) and configured with CMS cytoplasm or restorer genes. This preliminary preparation allows the actual hybrid crossing to be a single-step operation that immediately produces F1 hybrids with all desired traits combined, dramatically reducing the time required compared to traditional multi-generational breeding approaches.

Inventive Principle:
Principle #10Preliminary action

Solution Approach 2:

The patent segments the breeding process into distinct functional components: (1) development of inbred parental lines with specific traits, (2) configuration of CMS cytoplasm and nuclear gene combinations, and (3) single-step hybrid production. This segmentation allows each component to be optimized independently and combined efficiently. By dividing the complex breeding task into manageable segments with clear functions, the overall breeding time is reduced while ensuring proper combination of desirable traits in the final hybrid.

Inventive Principle:
Principle #1Segmentation

Data Source

PatentUS10321649B1Plants and seeds of hybrid corn variety CH267703
Publication Date: 2019.06.18 MONSANTO TECHNOLOGY LLC

AI summary

According to the invention, there is provided seed and plants of the hybrid corn variety designated CH267703. The invention thus relates to the plants, seeds and tissue cultures of the variety CH267703, and to methods for producing a corn plant produced by crossing a corn plant of variety CH267703 with itself or with another corn plant, such as a plant of another variety. The invention further relates to genetic complements of plants of variety CH267703.