Hybrid Corn CH555099 Breeding for Genetic Uniformity

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Solution Overview

Problem

Current corn breeding techniques face challenges in developing uniform hybrid varieties with desirable traits such as high yield, disease resistance, and uniform germination due to genetic non-uniformity and unpredictability in cross-pollination, necessitating the development of stable inbred plants with specific desirable characteristics.

Innovation Solution

The development of the hybrid corn variety CH555099, which includes genetic loci for traits like male sterility, herbicide resistance, and disease resistance, utilizing cytoplasmic-male sterility and transgenes integrated at specific chromosomal locations, along with tissue cultures capable of regenerating plants with consistent physiological and morphological characteristics.

Engineering Contradictions & Design Principles

VSEngineering Contradiction Analysis

1Adaptability or versatility

If cross-pollination is used to develop corn hybrids, then genetic diversity and trait combination are improved, but genetic uniformity and predictability deteriorate

Engineering Contradiction:
Improvegenetic diversityVSAvoidgenetic uniformity
Core Design Contradiction:
Adaptability or versatilityVSStability of the object's composition

Solution Approach 1:

The breeding process is segmented into distinct phases: first developing homozygous inbred lines through self-pollination to ensure genetic uniformity, then crossing these standardized lines to introduce controlled genetic diversity. This segmentation allows each phase to optimize for its specific goal without compromising the other.

Inventive Principle:
Principle #1Segmentation

Solution Approach 2:

Homozygous inbred parent lines are developed and standardized before the hybrid crossing stage. This preliminary action ensures that the genetic composition of parents is fixed and uniform, which then enables predictable and uniform hybrid offspring when crossing occurs.

Inventive Principle:
Principle #10Preliminary action

2Stability of the object's composition

If self-pollination is used to develop inbred lines, then genetic uniformity is improved, but genetic diversity and adaptability deteriorate

Engineering Contradiction:
Improvegenetic uniformityVSAvoidgenetic diversity
Core Design Contradiction:
Stability of the object's compositionVSAdaptability or versatility

Solution Approach 1:

Self-pollination is used as a preliminary action to fix the genetic composition of parent lines before crossing. This creates genetically uniform inbreds that serve as standardized building blocks for subsequent hybridization, ensuring predictability in the final hybrid product.

Inventive Principle:
Principle #10Preliminary action

Solution Approach 2:

Multiple inbred lines, each developed through self-pollination for uniformity, are merged through cross-pollination to create hybrids that combine the desirable traits of each parent while maintaining uniformity across the hybrid population.

Inventive Principle:
Principle #5Merging (Combining)

3Adaptability or versatility

If traditional breeding methods are used, then natural genetic variation is preserved, but breeding precision and trait predictability deteriorate

Engineering Contradiction:
Improvenatural genetic variationVSAvoidbreeding precision
Core Design Contradiction:
Adaptability or versatilityVSManufacturing precision

Solution Approach 1:

Homozygous inbred lines serve as intermediaries between natural genetic variation and the final hybrid product. These inbreds capture and fix specific genetic variations in a uniform, standardized form that can be precisely combined with other inbreds to achieve predictable hybrid outcomes.

Inventive Principle:
Principle #24Intermediary (Mediator)

Solution Approach 2:

The breeding approach changes the state of genetic parameters by fixing allele frequencies to homozygosity in parent lines through self-pollination. This parameter change from heterozygous to homozygous states enables precise control over which traits are combined in the hybrid generation.

Inventive Principle:
Principle #35Parameter changes

Data Source

PatentUS9265217B2Plants and seeds of hybrid corn variety CH555099
Publication Date: 2016.02.23 MONSANTO TECHNOLOGY LLC

AI summary

According to the invention, there is provided seed and plants of the hybrid corn variety designated CH555099. The invention thus relates to the plants, seeds and tissue cultures of the variety CH555099, and to methods for producing a corn plant produced by crossing a corn plant of variety CH555099 with itself or with another corn plant, such as a plant of another variety. The invention further relates to genetic complements of plants of variety CH555099.