Corn Haploid Induction Line Hybridization Strategy
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Solution Overview
Problem
Current corn haploid induction lines, whether temperate or tropical, fail to meet the requirements for adaptability, pollen quantity, plant height, and growth period when used on a large scale in corn breeding, leading to inefficiencies and high costs in breeding corn inbred lines.
Innovation Solution
A method involving hybridization between a tropical induction line with strong disease resistance and a temperate induction line to generate hybrid seeds, which are then used as a male parent in a 'seed production' manner to enhance induction efficiency, combining the hybrid vigor for improved traits such as pollen quantity, disease resistance, and plant height.
Engineering Contradictions & Design Principles
Engineering Contradiction Analysis
1Productivity
If a temperate induction line (e.g., CAU5) is used as male parent, then induction rate and pollen quantity are improved, but adaptability to tropical and subtropical regions deteriorates
Solution Approach 1:
The patent merges the advantages of temperate induction lines (high induction rate, large pollen quantity) with tropical induction lines (strong adaptability, disease resistance) by creating a hybrid induction line. The hybrid line combines the positive traits of both parents, achieving both high productivity and adaptability to tropical environments simultaneously.
Solution Approach 2:
The patent changes the genetic parameters of the induction line by hybridizing temperate and tropical lines. This genetic parameter change results in a new hybrid induction line that exhibits different characteristics from either parent, specifically achieving both high induction rate and strong adaptability to tropical regions.
2Adaptability or versatility
If a tropical induction line (e.g., Yun induction No. 2) is used as male parent, then adaptability and disease resistance are improved, but plant height and pollen quantity deteriorate
Solution Approach 1:
The patent merges the advantages of tropical induction lines (strong adaptability, disease resistance) with temperate induction lines (large pollen quantity, high plant height) by creating a hybrid induction line. The hybrid line combines the positive traits of both parents, achieving both high productivity and adaptability to tropical environments simultaneously.
3Reliability
If traditional inbred line breeding is used, then genetic purity is maintained, but breeding time and cost increase significantly
Solution Approach 1:
The patent creates a hybrid induction line in advance that possesses the desired traits (high induction rate, large pollen quantity, strong adaptability). This preliminary creation of a specialized induction line enables subsequent efficient haploid induction without requiring lengthy traditional inbreeding processes, thus reducing breeding time while maintaining genetic purity.
Solution Approach 2:
The patent changes the breeding approach from traditional multi-generation inbreeding to a accelerated haploid induction method using a specially bred hybrid induction line. This parameter change in the breeding process reduces the time required to obtain pure inbred lines while maintaining genetic integrity through controlled hybridization and haploid induction.
Data Source
AI summary
A method for enhancing induction efficiency of corn haploid is disclosed, which belongs to the technical field of genetics. In the method disclosed by the disclosure, the tropical induction line is used as a female parent, the temperate induction line is used as a male parent, hybrid seeds among the temperate and tropical induction lines are generated by hybridizing and assembling, the hybrid vigor among the temperate and tropical induction is used for improving the agronomy and yield traits of the conventional induction line, so that the requirements of the corn haploid induction line as the male parent on plant height, pollen quantity, fertility period and the like are met, which has important significance for large-scale popularization and application of the corn double haploid (DH) technology.