Corn Variety I168643 Breeding for Genetic Uniformity

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Solution Overview

Problem

Corn breeding aims to combine desirable traits like yield, disease resistance, and uniformity, but existing methods struggle to produce stable inbred plants with predictable performance due to genetic non-uniformity from cross-pollination, leading to unpredictable hybrid performance.

Innovation Solution

The development of the corn variety I168643, which includes cytoplasmic or nuclear factors for male sterility and additional heritable traits like herbicide tolerance and disease resistance, along with tissue cultures capable of regenerating plants with specific physiological and morphological characteristics, ensures a homogeneous population of inbred plants and hybrid production through controlled pollination processes.

Engineering Contradictions & Design Principles

VSEngineering Contradiction Analysis

1Adaptability or versatility

If cross-pollination is used to combine desirable traits from different plants, then genetic diversity and trait combination are improved, but genetic uniformity and predictability of hybrid performance deteriorate

Engineering Contradiction:
Improvetrait combinationVSAvoidgenetic uniformity
Core Design Contradiction:
Adaptability or versatilityVSStability of the object's composition

Solution Approach 1:

The breeding process is segmented into distinct phases: first developing homozygous inbred lines through repeated self-pollination to achieve genetic uniformity, then crossing these standardized inbreds to produce uniform hybrids. This segmentation allows each phase to optimize for its specific goal without compromising the other.

Inventive Principle:
Principle #1Segmentation

Solution Approach 2:

Inbred parental lines are developed and standardized through multiple generations of self-pollination and selection before the actual hybrid crossing. This preliminary action ensures that the parental lines are genetically uniform and stable, which then enables predictable hybrid performance when crossed.

Inventive Principle:
Principle #10Preliminary action

2Stability of the object's composition

If self-pollination is used to develop homozygous inbred plants, then genetic uniformity and true breeding are improved, but genetic diversity and trait combination are worsened

Engineering Contradiction:
Improvegenetic uniformityVSAvoidtrait combination
Core Design Contradiction:
Stability of the object's compositionVSAdaptability or versatility

Solution Approach 1:

Self-pollination and selection are performed as a preliminary action to develop standardized homozygous inbred lines with desired traits. Once these uniform inbreds are established, they serve as parental stocks for subsequent crossing to combine traits from different inbred lines.

Inventive Principle:
Principle #10Preliminary action

Solution Approach 2:

Homozygous inbred lines serve as an intermediary between the diverse germplasm pool and the final hybrid varieties. The inbreds standardize and stabilize genetic material before it is combined in hybrids, acting as a bridge that enables predictable trait combination.

Inventive Principle:
Principle #24Intermediary (Mediator)

3Ease of manufacture

If natural pollination is used in corn, then breeding simplicity is improved, but control over pollination and genetic uniformity are worsened

Engineering Contradiction:
Improvebreeding simplicityVSAvoidgenetic uniformity
Core Design Contradiction:
Ease of manufactureVSStability of the object's composition

Solution Approach 1:

The tassel (male reproductive structure) is removed from selected female parental plants to prevent self-pollination and contamination. This extraction of the male organ ensures that only controlled cross-pollination from designated male parents occurs, maintaining genetic uniformity in the hybrid seed production.

Inventive Principle:
Principle #2Taking out (Extraction)

4Stability of the object's composition

If repeated selfing and selection are used to develop inbred plants, then homozygosity and uniformity are improved, but breeding time and complexity are worsened

Engineering Contradiction:
ImprovehomozygosityVSAvoidbreeding time
Core Design Contradiction:
Stability of the object's compositionVSLoss of time

Solution Approach 1:

The inbreeding process follows a periodic pattern of repeated self-pollination over multiple generations (typically 6-8 generations), with systematic selection at each stage. This periodic application of selfing and selection efficiently accumulates homozygosity without requiring excessive time by maintaining a consistent breeding rhythm.

Inventive Principle:
Principle #19Periodic action

Data Source

PatentUS7388139B1Plants and seeds of corn variety I168643
Publication Date: 2008.06.17 MONSANTO TECHNOLOGY LLC

AI summary

According to the invention, there is provided seed and plants of the corn variety designated I168643. The invention thus relates to the plants, seeds and tissue cultures of the variety I168643, and to methods for producing a corn plant produced by crossing a corn plant of variety I168643 with itself or with another corn plant, such as a plant of another variety. The invention further relates to corn seeds and plants produced by crossing plants of variety I168643 with plants of another variety, such as another inbred line. The invention further relates to the inbred and hybrid genetic complements of plants of variety I168643.