Inbred Corn Line 0EEIX5165 Breeding Program

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Solution Overview

Problem

The development of new inbred corn lines is a slow and costly process that requires expertise, and existing inbred parent corn lines lose commercial competitiveness over time, necessitating the creation of improved germplasm with enhanced yield, disease resistance, and adaptability.

Innovation Solution

The introduction of the inbred corn line 0EEIX5165, which is a yellow dent inbred corn line with specific physiological and morphological characteristics, and methods for producing and breeding corn plants using techniques such as self-pollination, sib-pollination, and tissue culture to create uniform and stable inbred and hybrid corn plants, along with the use of genetic markers and mutagenesis to introduce desired traits.

Engineering Contradictions & Design Principles

VSEngineering Contradiction Analysis

1Stability of the object's composition

If traditional inbreeding techniques are used to develop new inbred corn lines, then genetic uniformity and purity are improved, but the development process becomes slow and costly

Engineering Contradiction:
Improvegenetic uniformityVSAvoiddevelopment time
Core Design Contradiction:
Stability of the object's compositionVSLoss of time

Solution Approach 1:

The patent establishes a structured multi-generation inbreeding program that performs preliminary actions in advance: developing inbred lines through systematic self-pollination and selection over multiple generations (S1-S5), pre-testing lines for desired traits, and preparing standardized breeding protocols before commercial deployment. This preliminary structured approach accelerates the overall development timeline while maintaining genetic purity.

Inventive Principle:
Principle #10Preliminary action

Solution Approach 2:

The patent implements parameter changes by transitioning from traditional random inbreeding to a controlled system with specific generation markers (S1-S5), standardized selection criteria for yield and disease resistance, and defined pollination protocols. These parameter standardizations streamline the breeding process, reducing variability and accelerating line development while maintaining genetic uniformity.

Inventive Principle:
Principle #35Parameter changes

2Productivity

If existing inbred parent corn lines are used repeatedly, then breeding efficiency is maintained, but commercial competitiveness decreases over time

Engineering Contradiction:
Improvebreeding efficiencyVSAvoidcommercial competitiveness
Core Design Contradiction:
ProductivityVSReliability

Solution Approach 1:

The patent implements a dynamic inbred line development program that continuously introduces new genetic material through repeated crosses and selections. Instead of statically using existing lines, the system dynamically generates new inbred lines (0EEIX5165, 0EEIX5166, etc.) with improved traits, ensuring ongoing commercial competitiveness while maintaining breeding efficiency through standardized protocols.

Inventive Principle:
Principle #15Dynamics

Solution Approach 2:

The patent systematically discards inferior inbred lines that lose competitiveness and recovers valuable traits by introducing new genetic material through controlled crosses. The program evaluates multiple lines across generations and retains only those demonstrating superior yield, disease resistance, and adaptability, ensuring the breeding program continuously refreshes its genetic base while maintaining efficiency.

Inventive Principle:
Principle #34Discarding and recovering

3Reliability

If intensive inbreeding is performed to develop superior lines, then yield and disease resistance are improved, but line vigor decreases

Engineering Contradiction:
Improvedisease resistanceVSAvoidline vigor
Core Design Contradiction:
ReliabilityVSStrength

Solution Approach 1:

The patent maintains continuous useful action by implementing an ongoing multi-generation inbreeding program (S1-S5) that continuously selects for both disease resistance and vigor. Rather than performing intensive inbreeding to completion, the system continuously refines lines across generations, maintaining vigor through selective breeding while progressively improving disease resistance and yield traits.

Inventive Principle:
Principle #20Continuity of useful action

Solution Approach 2:

The patent applies partial inbreeding action by performing a sufficient number of generations (S1-S5) to establish disease resistance and purity, but not so many generations that vigor is completely depleted. The program identifies the optimal stopping point where lines have adequate disease resistance and uniformity but retain sufficient vigor for commercial production, avoiding excessive inbreeding depression.

Inventive Principle:
Principle #16Partial or excessive action

4Reliability

If manual detasseling is used to produce hybrid seed, then hybrid purity is improved, but labor costs and production time increase

Engineering Contradiction:
Improvehybrid purityVSAvoidseed production efficiency
Core Design Contradiction:
ReliabilityVSProductivity

Solution Approach 1:

The patent implements self-service by developing and utilizing male-sterile inbred lines that automatically prevent self-pollination and enable hybrid seed production without manual detasseling. The male-sterile lines naturally produce no viable pollen, allowing female parent lines to be pollinated exclusively by male parent lines, ensuring hybrid purity while eliminating labor-intensive detasseling operations and improving seed production efficiency.

Inventive Principle:
Principle #25Self-service

Data Source

PatentUS10470409B1Inbred corn line 0EEIX5165
Publication Date: 2019.11.12 AGRIGENETICS INC

AI summary

An inbred corn line, designated 0EEIX5165, the plants and seeds of the inbred corn line 0EEIX5165, methods for producing a corn plant, either inbred or hybrid, produced by crossing the inbred corn line 0EEIX5165 with itself or with another corn plant, and hybrid corn seeds and plants produced by crossing the inbred line 0EEIX5165 with another corn line or plant and to methods for producing a corn plant containing in its genetic material one or more transgenes and to the transgenic corn plants produced by that method. This invention also relates to inbred corn lines derived from inbred corn line 0EEIX5165, to methods for producing other inbred corn lines derived from inbred corn line 0EEIX5165 and to the inbred corn lines derived by the use of those methods.