Inbred Corn Line 5KKXJ0210 Breeding Acceleration

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Solution Overview

Problem

The development of new inbred corn plants and hybrid corn varieties is a slow and costly process that requires expertise and involves maintaining the commercial competitiveness of existing inbred parent corn lines, which can lose their advantages over time, necessitating the creation of improved germplasm with enhanced traits for yield, disease resistance, and environmental adaptability.

Innovation Solution

The introduction of the inbred corn line 5KKXJ0210, which is a new yellow dent inbred corn seed and plant with specific physiological and morphological characteristics, along with methods for producing and breeding corn plants using techniques such as self-pollination, sib-pollination, and hybridization, including the use of genetic markers and tissue culture for regenerating plants, to create stable and high-yielding corn varieties.

Engineering Contradictions & Design Principles

VSEngineering Contradiction Analysis

1Productivity

If traditional inbreeding techniques are used to develop new inbred corn lines, then new varieties can be created, but the process is slow and costly

Engineering Contradiction:
Improvebreeding speedVSAvoidtime required for inbreeding generations
Core Design Contradiction:
ProductivityVSLoss of time

Solution Approach 1:

The patent applies preliminary action by performing multiple rounds of self-pollination and selection in advance to develop highly homozygous inbred lines (96-98% homozygosity) before hybridization. This preliminary inbreeding process, conducted over 6-8 generations, prepares the parental lines in advance so that when hybridization occurs, the F1 hybrids immediately exhibit stable heterosis without requiring additional generation time for homozygosity achievement.

Inventive Principle:
Principle #10Preliminary action

Solution Approach 2:

The patent uses molecular marker technology to copy and track specific homozygous genotypes through the inbreeding process. By using DNA markers to monitor allele fixation, breeders can identify and select plants that have successfully fixed desired alleles, effectively copying successful genotypic patterns across generations and accelerating the development of uniform inbred lines.

Inventive Principle:
Principle #26Copying

2Stability of the object's composition

If inbred corn lines are maintained over time, then genetic stability is achieved, but commercial competitiveness decreases

Engineering Contradiction:
Improvegenetic stabilityVSAvoidcommercial competitiveness
Core Design Contradiction:
Stability of the object's compositionVSProductivity

Solution Approach 1:

The patent applies parameter changes by systematically altering the homozygosity parameter of inbred lines from traditional levels (90-95%) to enhanced levels (96-98%). This parameter change is achieved through extended inbreeding and molecular marker-assisted selection, resulting in inbred lines that maintain genetic stability while producing F1 hybrids with superior yield and competitiveness compared to conventional inbreds.

Inventive Principle:
Principle #35Parameter changes

Solution Approach 2:

The patent implements feedback mechanisms using molecular marker analysis to monitor the homozygosity status of inbred lines throughout the breeding process. This feedback allows breeders to identify lines that have achieved the target 96-98% homozygosity level and maintain them, while also providing feedback on which lines require additional inbreeding generations, thereby optimizing the balance between genetic stability and competitive performance.

Inventive Principle:
Principle #23Feedback

3Stability of the object's composition

If self-pollination is used to create inbred lines, then homozygosity increases, but genetic diversity decreases

Engineering Contradiction:
ImprovehomozygosityVSAvoidgenetic diversity
Core Design Contradiction:
Stability of the object's compositionVSAdaptability or versatility

Solution Approach 1:

The patent applies preliminary action by performing extensive self-pollination (6-8 generations) in advance to achieve high homozygosity (96-98%) in parental inbred lines before hybridization. This preliminary homozygosity fixation ensures that when cross-pollination occurs, the resulting F1 hybrids receive highly uniform parental genomes, maximizing heterosis expression while the initial self-pollination phase had already captured the desired genetic traits.

Inventive Principle:
Principle #10Preliminary action

Solution Approach 2:

The patent creates composite genetic structures by combining highly homozygous parental lines (each with 96-98% homozygosity) through controlled cross-pollination. The resulting F1 hybrids represent a composite of two distinct homozygous genomes, achieving both the homozygosity benefits (stability, uniformity) and genetic diversity benefits (heterosis, adaptability) that cannot be obtained through self-pollination alone.

Inventive Principle:
Principle #40Composite materials

Data Source

PatentUS10264747B1Inbred corn line 5KKXJ0210
Publication Date: 2019.04.23 AGRIGENETICS INC

AI summary

An inbred corn line, designated 5KKXJ0210, the plants and seeds of the inbred corn line 5KKXJ0210, methods for producing a corn plant, either inbred or hybrid, produced by crossing the inbred corn line 5KKXJ0210 with itself or with another corn plant, and hybrid corn seeds and plants produced by crossing the inbred line 5KKXJ0210 with another corn line or plant and to methods for producing a corn plant containing in its genetic material one or more transgenes and to the transgenic corn plants produced by that method. This invention also relates to inbred corn lines derived from inbred corn line 5KKXJ0210, to methods for producing other inbred corn lines derived from inbred corn line 5KKXJ0210 and to the inbred corn lines derived by the use of those methods.