Inbred Corn Line LK18838N1 Breeding Efficiency

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Solution Overview

Problem

The challenge in corn breeding is to develop stable, high-yielding corn hybrids with superior parental lines that exhibit desirable traits such as disease resistance, insect tolerance, and improved nutritional quality, while maintaining genetic stability and uniformity across different environmental conditions.

Innovation Solution

The development of the inbred corn line LK18838N1, which can be used to produce F1 hybrid seeds and plants with specific traits through crossing and backcrossing methods, incorporating transgenes for traits like herbicide resistance, insect resistance, and improved nutritional quality, and utilizing mutagenesis techniques like TILLING for genetic modification.

Engineering Contradictions & Design Principles

VSEngineering Contradiction Analysis

1Productivity

If conventional breeding methods are used to develop corn hybrids, then genetic diversity can be maintained, but breeding time and complexity increase significantly

Engineering Contradiction:
Improvebreeding efficiencyVSAvoidbreeding time
Core Design Contradiction:
ProductivityVSLoss of time

Solution Approach 1:

The patent employs mutagenesis techniques (chemical, physical, or biological) to induce controlled genetic changes in the corn inbred line. This accelerates the breeding process by creating desired traits directly rather than relying on slow natural mutation and selection, thus resolving the contradiction between breeding efficiency and time consumption

Inventive Principle:
Principle #35Parameter changes

Solution Approach 2:

The patent uses TILLING (Targeting Induced Local Lesions in Genomes) as an intermediary screening method to efficiently identify plants with desired mutations. This mediator technique allows rapid detection of specific genetic changes, significantly reducing the time required to develop stable high-yielding hybrids

Inventive Principle:
Principle #24Intermediary (Mediator)

2Productivity

If multiple traits are incorporated into corn hybrids, then agronomic performance improves, but genetic stability becomes more difficult to maintain

Engineering Contradiction:
Improveagronomic performanceVSAvoidgenetic stability
Core Design Contradiction:
ProductivityVSStability of the object's composition

Solution Approach 1:

The patent performs preliminary mutagenesis and trait incorporation during the inbred line development stage (before hybrid production). By establishing the desired traits and genetic stability in the parental inbred line LK18838N1 first, the resulting F1 hybrids automatically inherit stable, uniform expression of multiple traits without the complexity of maintaining them through additional breeding steps

Inventive Principle:
Principle #10Preliminary action

Solution Approach 2:

The patent applies mutagenesis and selection to specific local regions of the genome to introduce desired traits (disease resistance, insect tolerance, nutritional quality) while maintaining the integrity and stability of other genomic regions. This localized approach allows multiple traits to be incorporated without compromising overall genetic stability

Inventive Principle:
Principle #3Local quality

3Productivity

If mutagenesis techniques are used for genetic modification, then trait development accelerates, but off-target mutations may occur

Engineering Contradiction:
Improvetrait development speedVSAvoidoff-target mutations
Core Design Contradiction:
ProductivityVSObject-affected harmful factors

Solution Approach 1:

The patent employs TILLING as a feedback screening mechanism to identify and select only those plants with desired on-target mutations while eliminating plants with off-target mutations. This feedback loop allows rapid trait development by continuously monitoring and selecting for precise genetic changes, thus resolving the contradiction between development speed and mutation accuracy

Inventive Principle:
Principle #23Feedback

Data Source

PatentUS12150423B2Inbred corn line LK18838N1
Publication Date: 2024.11.26 KWS SAAT SE & CO KGAA

AI summary

Inbred corn line, designated LK18838N1, are disclosed. The disclosure relates to the seeds of inbred corn line LK18838N1, to the plants and plant parts of inbred corn line LK18838N1 and to methods for producing a corn plant, either inbred or hybrid, by crossing inbred corn line LK18838N1 with itself or another corn line. The disclosure also relates to products produced from the seeds, plants, or parts thereof, of inbred corn line LK18838N1 and/or of the hybrids produced using the inbred as a parent. The disclosure further relates to methods for producing a corn plant containing in its genetic material one or more transgenes and to the transgenic plants produced by that method and to methods for producing other corn lines derived from inbred corn line LK18838N1.