Dual Detection Kit for Novel Coronavirus Nucleic Acid
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Solution Overview
Problem
Current methods for diagnosing novel coronavirus infection, such as CT lung imaging, face challenges including cross-infection risks, potential misdiagnosis, and high equipment costs, necessitating a more efficient and cost-effective detection method.
Innovation Solution
A primer and probe combination kit for real-time fluorescence PCR that simultaneously detects the ORF1ab and N genes of the novel coronavirus, incorporating internal standard primers and probes to enhance specificity and accuracy, and includes a PCR enzyme system and quality control products for reliable nucleic acid detection.
Engineering Contradictions & Design Principles
Engineering Contradiction Analysis
1Reliability
If CT lung imaging is used for diagnosis, then detection capability is provided, but cross-infection risk increases and equipment cost increases
Solution Approach 1:
The patent replaces the mechanical CT imaging system with a biochemical nucleic acid detection system (real-time fluorescence PCR). This substitution eliminates the need for expensive CT equipment and reduces cross-infection risks by using sealed reaction tubes with integrated sampling and detection, thereby resolving the contradiction between detection capability and cross-infection risk.
2Reliability
If CT lung imaging is used for diagnosis, then detection capability is provided, but misdiagnosis possibility increases
Solution Approach 1:
The patent replaces non-specific CT imaging with specific nucleic acid detection that targets coronavirus genetic material. This biochemical substitution provides molecular-level specificity through primer-probe combinations that uniquely identify coronavirus sequences, eliminating the ground glass shadow ambiguity of CT and dramatically improving diagnosis accuracy.
3Reliability
If CT instruments are deployed, then detection capability is improved, but equipment cost increases and accessibility decreases
Solution Approach 1:
The patent employs disposable sealed reaction tubes containing pre-loaded reagents for nucleic acid detection. This replaces expensive, complex CT instruments with inexpensive, single-use detection systems that can be deployed in resource-limited areas, thereby resolving the contradiction between detection capability and equipment cost.
Solution Approach 2:
The patent substitutes expensive CT imaging equipment with affordable biochemical detection systems using standard laboratory equipment. This mechanical-to-biochemical substitution dramatically reduces equipment costs while maintaining detection capability, enabling deployment in remote and underdeveloped areas.
4Ease of operation
If single-target detection is used, then detection simplicity is maintained, but detection accuracy decreases
Solution Approach 1:
The patent merges multiple detection targets (ORF1ab gene and N gene) into a single real-time fluorescence PCR reaction system. This allows simultaneous dual-target detection in one test, maintaining operational simplicity while significantly improving accuracy through mutual verification to prevent false positives and negatives.
Applied Scientific Principles
This section explains which scientific principles are used to turn an abstract innovation direction into a practical engineering solution.
Function Achieved in This Case
The kit provides high sensitivity, specificity, and repeatability for detecting novel coronavirus nucleic acid, reducing false positives and negatives, and is suitable for resource-limited areas, offering a reliable basis for virus identification and control.
Implementation Method 1
The probe is an oligonucleotide, and a fluorescent reporter group and a fluorescent quenching group are labeled at both ends. When the probe is complete, the fluorescent signal emitted by the reporter group is absorbed by the quenching group. At the beginning, the probe is bound to any single strand of DNA and during PCR amplification, the 5'-3' exonuclease activity of the Taq enzyme degrade the probe by the digestion, so that the fluorescent reporter group and the fluorescent quenching group are separated. Then the fluorescence monitoring system can receive the fluorescent signal.
Data Source
AI summary
The present invention provides a novel coronavirus duplex detection kit. Specifically, the present invention discloses a kit and method for multiplex detection of novel coronavirus 2019-nCoV nucleic acid, which can simultaneously detect two nucleic acid targets of the novel coronavirus 2019-nCoV and possess extremely high sensitivity and specificity, and significantly improve the accuracy of virus identification.