Cotton Variety FM 9160B2F Breeding Segmentation

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Solution Overview

Problem

Cotton breeding faces challenges in predicting phenotypic expression due to the complexity of gene structures and environments, making it difficult to reproduce consistent varieties, and existing methods lack efficiency in introducing desired traits while preserving unique characteristics.

Innovation Solution

The development of the cotton variety FM 9160B2F and its hybrid varieties through a breeding process involving selection, self-fertilization, and recurrent backcrossing, combined with genetic transformation methods like Agrobacterium infection and microprojectile bombardment, to produce seeds and plants with specific traits such as herbicide resistance and insect resistance.

Engineering Contradictions & Design Principles

VSEngineering Contradiction Analysis

1Stability of the object's composition

If traditional breeding methods are used to preserve plant characteristics, then variety stability is maintained, but the ability to introduce new traits is limited

Engineering Contradiction:
Improvevariety stabilityVSAvoidability to introduce new traits
Core Design Contradiction:
Stability of the object's compositionVSAdaptability or versatility

Solution Approach 1:

The breeding program segments the breeding process into distinct phases: initial variety development through self-pollination, followed by separate backcrossing phases for introducing specific traits (herbicide resistance, insect resistance), and final selection phases. This segmentation allows preservation of the original variety's stable characteristics while systematically introducing new traits through controlled crosses with specific parent lines.

Inventive Principle:
Principle #1Segmentation

2Productivity

If genetic transformation methods are used to introduce desired traits, then trait introduction efficiency is improved, but the complexity of gene structure prediction becomes more challenging

Engineering Contradiction:
Improvetrait introduction efficiencyVSAvoidgene structure complexity
Core Design Contradiction:
ProductivityVSDevice complexity

Solution Approach 1:

The patent uses Agrobacterium tumefaciens as an intermediary organism to mediate the transfer of desired traits (herbicide resistance genes, insect resistance genes) into the cotton plant genome. This biological mediator simplifies the complex process of genetic transformation by providing a natural vector for DNA transfer, making the introduction of multiple traits more efficient despite the underlying genomic complexity.

Inventive Principle:
Principle #24Intermediary (Mediator)

3Manufacturing precision

If recurrent backcrossing is performed to derive new varieties, then genetic conformity with the initial variety is improved, but the breeding time and generations required increase

Engineering Contradiction:
Improvegenetic conformityVSAvoidbreeding time
Core Design Contradiction:
Manufacturing precisionVSLoss of time

Solution Approach 1:

The breeding program performs preliminary actions by first establishing a stable initial variety (FM 9160B2F) with desired baseline characteristics through self-pollination and selection. This pre-established genetic foundation allows subsequent backcrossing operations to more efficiently achieve genetic conformity, as the recurrent parent's stable genome provides a consistent target for selection, reducing the number of generations needed compared to starting from variable parent lines.

Inventive Principle:
Principle #10Preliminary action

Data Source

PatentUS8912403B2Cotton variety FM 9160B2F
Publication Date: 2014.12.16 BASF AGRICULTURAL SOLUTIONS US LLC

AI summary

The cotton variety FM 9160B2F is disclosed. The invention relates to seeds, plants, plant cells, plant tissue, harvested products and cotton lint as well as to hybrid cotton plants and seeds obtained by repeatedly crossing plants of variety FM 9160B2F with other plants. The invention also relates to plants and varieties produced by the method of essential derivation from plants of FM 9160B2F and to plants of FM 9160B2F reproduced by vegetative methods, including but not limited to tissue culture of regenerable cells or tissue from FM 9160B2F.