Cell Sheet Detachment With Cross-Directional Tapping and Shaking
Find Innovative SolutionsGenerate Solutions
Solution Overview
Problem
Existing methods for detaching cell sheets from culture substrates often result in tearing and inefficiency, particularly when the direction of movement is parallel to the impact direction.
Innovation Solution
A combined method of tapping and shaking the culture container at angles relative to each other, utilizing external stimuli like tapping, shaking, and ultrasonic waves to detach cell sheets efficiently while minimizing tearing.
Engineering Contradictions & Design Principles
Engineering Contradiction Analysis
1Productivity
If the culture container is allowed to undergo back-and-forth movement in a direction parallel to the impact direction, then the cell sheet detachment efficiency is improved, but the cell sheet is prone to tearing
Solution Approach 1:
The patent changes the direction of movement from parallel to perpendicular relative to the impact direction. Specifically, the culture container is moved in a direction perpendicular to the impact direction applied by the detachment start unit, which prevents tearing while maintaining detachment efficiency. This dimensional change in movement direction resolves the contradiction between detachment efficiency and sheet integrity.
2Speed
If the culture container is tapped with a large impact force, then the detachment speed is increased, but the cell sheet is more likely to tear
Solution Approach 1:
The patent employs dynamic control of the tapping process through repeated, controlled impacts rather than a single large force. The detachment start unit repeatedly taps the culture container in a controlled manner, allowing gradual detachment while maintaining sheet integrity. This dynamic approach balances detachment speed with prevention of tearing.
3Productivity
If the culture container is shaken vigorously, then the detachment process is accelerated, but the cell sheet quality deteriorates
Solution Approach 1:
The patent uses periodic shaking movements rather than continuous vigorous shaking. The shaking unit performs repeated, controlled shaking cycles that gradually facilitate detachment while maintaining cell sheet quality. This periodic action allows the system to achieve high detachment efficiency without compromising sheet integrity through excessive force.
Applied Scientific Principles
This section explains which scientific principles are used to turn an abstract innovation direction into a practical engineering solution.
Function Achieved in This Case
The method effectively detaches cell sheets with reduced tearing and shorter detachment times, maintaining high cell survival rates and efficiency.
Implementation Method 1
a tapping process of tapping the culture container... the tapping unit that taps the culture container
Implementation Method 2
a shaking process of shaking the culture container... the shaking unit that shakes the culture container
Data Source
Figure 1
Figure 2
Figure 3
AI summary
A cell detachment method that detaches a cell sheet adhering to a culture surface of a culture container (8) from the culture container (8) by using a cell detachment system includes a tapping process of tapping the culture container (8) in a first direction that contains a direction component parallel to the culture surface; and a shaking process of shaking the culture container (8) in a second direction that contains a direction component parallel to the culture surface, in which the first direction and the second direction are different from each other as viewed in a direction perpendicular to the culture surface.