Cryopreserved Cell Recovery via Controlled Osmotic Dilution
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Solution Overview
Problem
Current methods for recovering living cells from cryopreserved cells are inefficient due to physical and chemical damages caused by freezing and thawing, leading to reduced cell viability and the need for prolonged cell proliferation to secure sufficient cells for transplantation.
Innovation Solution
A method involving the controlled dilution of thawed cell suspensions to manage osmotic pressure changes, with a maximum load of 40-50 mOsm/second, reduces cellular damage and increases cell survival ratios by minimizing abrupt osmotic shifts during the thawing process.
Engineering Contradictions & Design Principles
Engineering Contradiction Analysis
1Quantity of substance
If cells are cryopreserved to enable long-term storage and later use, then cell availability for transplantation is improved, but cell viability is reduced due to physical and chemical damage during freezing and thawing
Solution Approach 1:
The invention changes the parameter of osmotic pressure change rate during dilution, controlling it to 40-50 mOsm/second. This parameter optimization reduces cellular damage during thawing while maintaining cell viability and availability for transplantation.
2Speed
If conventional rapid thawing and dilution methods are used to minimize freezing damage, then cell recovery speed is improved, but cell viability is reduced due to abrupt osmotic pressure changes
Solution Approach 1:
The invention applies dynamic control to the dilution process, adjusting the rate of diluent addition to maintain osmotic pressure change within 40-50 mOsm/second. This dynamic optimization allows rapid thawing while preventing cellular damage from abrupt osmotic shifts.
Solution Approach 2:
The invention optimizes the osmotic pressure change rate parameter to 40-50 mOsm/second during dilution. This parameter control balances the speed of cell recovery with the maintenance of cell viability, avoiding the harm of both too-rapid and too-slow dilution.
3Quantity of substance
If extensive cell proliferation is performed after thawing to secure sufficient cells for transplantation, then cell quantity is improved, but preparation time is extended
Solution Approach 1:
The invention performs preliminary optimization during the thawing and dilution process itself, controlling osmotic pressure changes to maximize cell viability immediately after thawing. This preliminary action reduces the need for subsequent proliferation steps, thereby reducing preparation time while ensuring sufficient cell quantity.
Applied Scientific Principles
This section explains which scientific principles are used to turn an abstract innovation direction into a practical engineering solution.
Function Achieved in This Case
This approach enhances the recovery of viable cells, eliminating the need for extensive proliferation steps and ensuring sufficient cells for immediate use in sheet-like cell culture products for transplantation.
Implementation Method 1
diluting the thawed cell suspension with a diluent
Implementation Method 2
the maximum load of osmotic pressure at the time of dilution ranges from 40 mOsm/second to 50 mOsm/second
Data Source
AI summary
Disclosed herein are a method for recovering living cells highly efficiently from cryopreserved cells by thawing and a system designed for such a method. The method for recovering living cells from cryopreserved cells includes thawing cryopreserved cells and diluting the thawed cell suspension with a diluent, wherein the dilution is performed in such a way that the maximum load of osmotic pressure at the time of dilution is equal to or lower than 250 mOsm/second. The problem described above is solved by the method.