Cryopreservation Tool Concave Segmentation for Cell Restraint
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Solution Overview
Problem
Existing cryopreservation tools face difficulties in easily attaching and securely restraining living cells, such as embryos and ova, during the freezing process, leading to potential separation issues and inefficient freezing operations.
Innovation Solution
A cryopreservation tool with a body part and a living cell holding part made of cold-resistant materials, featuring a long and narrow attaching and holding portion with multiple concave portions for cell accommodation and excess cryopreservation liquid discharge passages, along with side bulged portions and projected features to prevent cell movement and ensure secure attachment.
Engineering Contradictions & Design Principles
Engineering Contradiction Analysis
1Reliability
If living cells are bonded to the inner surface of the cryopreservation container using minimum vitrifying liquid, then cell survival rate is improved and disease infection is prevented, but the operation becomes difficult and cells may separate during freezing
Solution Approach 1:
The cryopreservation container is divided into multiple concave portions (first, second, third concave portions) along its inner surface. Each concave portion can independently hold a living cell with minimal vitrifying liquid, allowing secure attachment while maintaining ease of operation. The segmentation enables cells to be positioned in discrete locations rather than requiring uniform bonding across the entire surface.
Solution Approach 2:
The concave portions act as intermediary structures that facilitate secure cell attachment. By providing defined geometric features (concave surfaces) on the container inner wall, the design creates natural holding positions that improve cell retention during freezing without requiring excessive bonding agents or complex operational procedures.
2Reliability
If living cells are securely restrained during freezing operation, then cell separation is prevented, but device complexity increases
Solution Approach 1:
The container inner surface is segmented into multiple concave portions that provide distributed restraint points for cells. This segmentation achieves reliable cell restraint across multiple locations without requiring a single complex restraint mechanism, thereby maintaining structural simplicity while improving overall reliability.
Solution Approach 2:
Instead of adding external restraint mechanisms to prevent cell separation, the design inverts the approach by creating concave portions that naturally accommodate and restrain cells through their geometric shape. The restraint function is built into the container structure itself rather than being added as a separate complexity-inducing component.
3Ease of operation
If multiple concave portions are formed for cell accommodation, then ease of operation is improved and cell placement becomes easier, but device complexity increases
Solution Approach 1:
The container is segmented into multiple concave portions that naturally guide and accommodate cells. This segmentation simplifies the cell placement operation by providing predefined locations for cells to settle, eliminating the need for precise manual positioning while maintaining a relatively simple overall device structure.
Solution Approach 2:
The concave portions enable cells to self-position and self-restrain within the container during the cryopreservation process. The geometric features of the concave portions automatically provide attachment surfaces and holding positions, reducing the operational complexity required for cell placement while improving ease of use.
Applied Scientific Principles
This section explains which scientific principles are used to turn an abstract innovation direction into a practical engineering solution.
Function Achieved in This Case
Facilitates easy operation for placing living cells, prevents cell separation during freezing, and allows for rapid freezing by managing excess cryopreservation liquid, ensuring high cell survival rates and efficient storage.
Implementation Method 1
The cryopreservation container is sealed and rapidly cooled by bringing the cryopreservation container into contact with liquid nitrogen
Implementation Method 2
rapidly cooled by bringing the cryopreservation container into contact with liquid nitrogen
Implementation Method 3
a plurality of excess cryopreservation liquid discharge passages communicating with the living cell accommodation concave portions
Data Source
AI summary
A living cell cryopreservation tool has a body part formed of a cold-resistant material and a living cell holding part formed of the cold-resistant material. The living cell holding part has a long and narrow living cell attaching and holding portion. The living cell attaching and holding portion has a plurality of living cell accommodation concave portions formed in a longitudinal direction thereof and a plurality of excess cryopreservation liquid discharge passages communicating with the living cell accommodation concave portions.