Cryo-EM Sample Preparation Device with Dual Jetting and Plunging Modes
Find Innovative SolutionsGenerate Solutions
Solution Overview
Problem
Existing cryo-electron microscope sample preparation devices are limited in their ability to handle a wide range of samples effectively, particularly in achieving consistent vitrification without damage.
Innovation Solution
A device with a holder for sample carriers, incorporating a jetting and plunging mechanism, allows for both jetting and plunging modes of cryogenic fluid application, enabling flexible sample preparation through a single transfer mechanism, including a control unit for operation control and user input for mode selection.
Engineering Contradictions & Design Principles
Engineering Contradiction Analysis
1Device complexity
If a single device structure is used for sample preparation, then device complexity is reduced, but adaptability to different sample types deteriorates
Solution Approach 1:
The device integrates both jetting and plunging functions into a single holder assembly. The holder can selectively position the sample carrier to receive either a jet of cryogenic fluid from a nozzle or be plunged into a reservoir of cryogenic fluid, enabling one device to perform multiple sample preparation modes for different sample types and requirements
2Adaptability or versatility
If multiple separate devices are used for jetting and plunging, then adaptability improves, but device complexity increases
Solution Approach 1:
The jetting nozzle and plunging reservoir are integrated into a single holder assembly. The holder simultaneously accommodates both the nozzle for jetting mode and the reservoir for plunging mode, allowing the device to provide both sample preparation methods through one unified structure rather than requiring separate devices
3Object-affected harmful factors
If the holder is positioned far from the cryogenic fluid source, then sample damage from premature cooling is reduced, but vitrification efficiency deteriorates
Solution Approach 1:
The holder is designed to be movable, allowing dynamic positioning of the sample carrier. The holder can be positioned at a distance from the cryogenic fluid source during transport to prevent premature cooling, then rapidly moved close to the source during the jetting or plunging operation to achieve efficient vitrification, thus adapting the position according to the operational phase
Applied Scientific Principles
This section explains which scientific principles are used to turn an abstract innovation direction into a practical engineering solution.
Function Achieved in This Case
The device provides enhanced flexibility and reliability in preparing samples by allowing both jetting and plunging modes, improving vitrification success rates and reducing sample damage.
Implementation Method 1
at least one jetting device (J) that is arranged for providing a jet of cryogenic fluid to said sample carrier (S)... The jetting device allows a jet or stream of cryogenic fluid to be brought into contact with the specimen provided onto the sample carrier so that the specimen may be vitrified
Implementation Method 2
the transfer mechanism may be arranged to function as the plunging device, by moving the sample carrier to the plunging position at an appropriate speed so that plunge freezing of the sample occurs
Implementation Method 3
a transfer mechanism that is operably coupled to the holder. The transfer mechanism is arranged for bringing the holder to a jetting position... The transfer mechanism is arranged for bringing the holder to a plunging position
Data Source
AI summary
A device for preparing samples for use in a cryo-electron microscope. The device comprises a holder for holding a sample carrier, at least one jetting device that is arranged for providing a jet of cryogenic fluid to said sample carrier, and a transfer mechanism that is operably coupled to the holder for bringing the holder to a jetting position. In the jetting position the holder is located near the at least one jetting device for receiving said jet of cryogenic fluid. The device further comprises a plunging reservoir for receiving a volume of cryogenic fluid, wherein the transfer mechanism is additionally arranged for bringing the holder to a plunging position. In the plunging position the holder is positioned at least partly inside an internal volume of said plunging reservoir for bringing the sample carrier in contact with cryogenic fluid.