Cryogenic storage system and method of transporting a sample in a cryogenic storage system

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Solution Overview

Problem

Current cryogenic storage systems lack efficient methods for organizing, tracking, and transferring samples between different cryogenic storage freezers and input/output ports, which can lead to inefficiencies and potential sample integrity issues due to manual handling and temperature control challenges.

Innovation Solution

A cryogenic storage system with a transfer module that includes a working chamber maintaining a cryogenic environment for sample tube transfer, a box transport robot for moving sample boxes between freezers and I/O ports, and a picker robot for transferring tubes between boxes, equipped with mechanisms to handle various sample box formats and maintain temperature control.

Engineering Contradictions & Design Principles

VSEngineering Contradiction Analysis

1Productivity

If manual handling of sample boxes is used in traditional cryogenic storage systems, then device complexity is reduced, but productivity and sample transfer efficiency deteriorate

Engineering Contradiction:
Improvesample transfer efficiencyVSAvoidsystem complexity
Core Design Contradiction:
ProductivityVSDevice complexity

Solution Approach 1:

The system employs automated robots that perform sample box transport and sample tube transfer operations independently without human intervention. The first robot autonomously navigates between storage freezers and the working chamber to transport sample boxes, while the second robot automatically transfers sample tubes between boxes within the working chamber, enabling the system to service itself

Inventive Principle:
Principle #25Self-service

Solution Approach 2:

Manual mechanical handling operations are replaced with automated robotic systems equipped with specialized end effectors. The robots use programmable control systems and automated gripping mechanisms to perform tasks previously requiring human hands, thereby increasing productivity while managing complexity through automation

Inventive Principle:
Principle #28Mechanics substitution (Replace mechanical system)

2Reliability

If sample boxes are frequently removed from cryogenic environment for manual access, then ease of operation improves, but sample integrity and temperature control deteriorate

Engineering Contradiction:
Improvesample integrityVSAvoidsample access convenience
Core Design Contradiction:
ReliabilityVSEase of operation

Solution Approach 1:

An automated robotic system acts as an intermediary between the user and the cryogenic storage environment. The robots perform all sample access operations through automated mechanisms, eliminating the need for users to manually remove sample boxes from the cryogenic environment. This maintains temperature stability and sample integrity while still providing access functionality

Inventive Principle:
Principle #24Intermediary (Mediator)

Solution Approach 2:

The system enables users to access samples through automated interfaces without physically entering or manipulating the cryogenic environment. The robotic systems handle all extraction, transfer, and return operations autonomously, allowing users to retrieve samples through simple commands while the samples remain protected in the controlled cryogenic environment throughout the process

Inventive Principle:
Principle #25Self-service

3Productivity

If automated robotic systems are implemented for sample transfer, then productivity improves, but device complexity and initial cost worsen

Engineering Contradiction:
Improvesample transfer efficiencyVSAvoidautomation system complexity
Core Design Contradiction:
ProductivityVSDevice complexity

Solution Approach 1:

The automated system is divided into distinct functional modules: a first robot dedicated to sample box transport between storage freezers and working chamber, a second robot dedicated to sample tube transfer within boxes, specialized end effectors for gripping, and a controlled working chamber. This segmentation allows each component to be optimized independently and simplifies maintenance and operation

Inventive Principle:
Principle #1Segmentation

Solution Approach 2:

The robotic systems are designed with universal capabilities to handle multiple sample box formats and configurations. The end effectors can adapt to different box types, and the robots can perform various operations including transport, positioning, and transfer tasks, reducing the need for specialized equipment for each function

Inventive Principle:
Principle #6Universality (Multi-functionality)

4Adaptability or versatility

If multiple sample box formats are accommodated, then adaptability improves, but device complexity and handling mechanism complexity worsen

Engineering Contradiction:
Improvesample box format compatibilityVSAvoidgripping mechanism complexity
Core Design Contradiction:
Adaptability or versatilityVSDevice complexity

Solution Approach 1:

The end effectors incorporate adjustable and reconfigurable gripping mechanisms that can dynamically adapt to different sample box formats. The gripping force, jaw position, and configuration can be modified programmatically to match the specific dimensions and features of various box types, allowing a single mechanism to handle multiple formats without requiring separate specialized grippers for each format

Inventive Principle:
Principle #15Dynamics

Applied Scientific Principles

This section explains which scientific principles are used to turn an abstract innovation direction into a practical engineering solution.

Function Achieved in This Case

Enables efficient and organized transfer of samples between cryogenic storage freezers and I/O ports, maintaining sample integrity by maintaining temperatures below the glass transition temperature, and accommodating various sample box formats, thus improving storage and retrieval processes.

Implementation Method 1

a working chamber (120) configured to maintain a cryogenic environment for transfer of sample tubes between different sample boxes

Methodology Applied
Scientific EffectCryogenic temperature maintenance: Cryogenics

Implementation Method 2

maintaining temperatures below the glass transition temperature

Methodology Applied
Scientific EffectGlass transition temperature control:

Implementation Method 3

The box transport robot may maintain an environment above a glass transition temperature of the samples being transported through the chamber

Methodology Applied
Scientific EffectTemperature control above glass transition:

Implementation Method 4

The ejector may include a pair of arms configured to clamp opposite sides of the first sample box. The ejector may also include a floor configured to support a sample box, where the floor includes one or more portions that are depressible via a force applied by the box transport robot

Methodology Applied
Scientific EffectMechanical force application: Mechanical Force

Data Source

PatentEP3768081B1Cryogenic storage system and method of transporting a sample in a cryogenic storage system
Publication Date: 2024.07.24 AZENTA US INC
  • EP3768081B1 patent drawingFigure 1A
  • EP3768081B1 patent drawingFigure 1B
  • EP3768081B1 patent drawingFigure 1C

AI summary

A cryogenic storage system (100) includes a transfer module (101) configured to service one or more cryogenic storage freezers (105a). The transfer module (101) includes a working chamber (120) that maintains a cryogenic environment for the transfer of sample tubes between different sample boxes. One or more freezer ports (108a) enable the transfer module (101) to receive a sample box extracted from a respective freezer (105a). An input/output (I/O) port (125) enables external access to samples. A box transport robot (130) operates to transport sample boxes between the freezer ports (108a), the working chamber (120), and the I/O port (125). A picker robot (140) operates to transfer sample tubes between sample boxes within the working chamber (120).