Crystalline 2'-Fucosyllactose Purification via Controlled Supersaturation
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Solution Overview
Problem
Current methods for obtaining crystalline 2′-fucosyllactose from fermentation processes are inefficient due to the need for chromatographic separation, which is costly and time-consuming, and often require organic solvents that are difficult to remove, especially when dealing with products containing significant amounts of mono- and oligosaccharides other than 2′-fucosyllactose.
Innovation Solution
A method involving controlled supersaturation of an aqueous solution of 2′-fucosyllactose raw material to selectively crystallize the compound without using excessive organic solvents, allowing for the separation of crystalline 2′-fucosyllactose in high purity and yield, and enabling the production of specific polymorphic forms by adjusting the crystallization temperature.
Engineering Contradictions & Design Principles
Engineering Contradiction Analysis
1Manufacturing precision
If chromatographic separation is used to purify 2'-fucosyllactose, then purity is improved, but production cost and time consumption increase
Solution Approach 1:
The invention changes the physical parameters of the system by controlling temperature and supersaturation levels to induce selective crystallization of 2'-fucosyllactose. By adjusting these parameters, the process achieves high purity separation without requiring chromatographic techniques, thereby reducing production costs and time while maintaining manufacturing precision
Solution Approach 2:
The invention utilizes phase transition through controlled crystallization from aqueous solution. By inducing supersaturation and controlling the phase transition from dissolved state to crystalline state, the process selectively precipitates 2'-fucosyllactose in high purity form, eliminating the need for chromatographic separation and reducing both time and cost
2Productivity
If organic solvents are used for crystallization, then crystallization efficiency is improved, but solvent removal difficulty increases
Solution Approach 1:
The invention changes the solvent system from organic solvents to aqueous solution and adjusts temperature parameters to achieve controlled supersaturation. This parameter change maintains crystallization efficiency while eliminating the problematic solvent removal step, as water can be easily removed by evaporation or drying without requiring additional purification steps
3Ease of manufacture
If fermentation process is used to produce 2'-fucosyllactose, then economic and environmental benefits are improved, but isolation complexity increases
Solution Approach 1:
The invention uses controlled crystallization phase transition from the fermentation broth directly, allowing 2'-fucosyllactose to selectively crystallize from the aqueous fermentation solution. This simple phase transition approach maintains the economic and environmental benefits of fermentation while dramatically simplifying the isolation process by eliminating complex chromatographic steps
Solution Approach 2:
The invention extracts 2'-fucosyllactose from the complex fermentation mixture through selective crystallization. By inducing controlled supersaturation, the target compound is selectively extracted in crystalline form while other fermentation components remain in solution, simplifying the isolation process while maintaining economic and environmental advantages
Applied Scientific Principles
This section explains which scientific principles are used to turn an abstract innovation direction into a practical engineering solution.
Function Achieved in This Case
This method achieves high-purity crystallization of 2′-fucosyllactose in high yield without organic solvents, allowing for the selective production of different polymorphic forms, thereby improving the efficiency and cost-effectiveness of the process.
Implementation Method 1
effecting the crystallization of 2'-FL from the solution provided in step a) by inducing conditions of a controlled supersaturation in the solution
Implementation Method 2
crystallization of 2'-FL, which has been prepared by classical organic synthesis, is an efficient means for isolation or purification of 2'-FL
Data Source
AI summary
The present invention relates to a method for obtaining crystalline 2′-fucosyllactose from a 2′-FL raw material, which contains 2′-FL as a main constituent and at least 0.5% by weight, frequently at least 1% by weight, in particular at least 2% by weight, more particularly at least 5% by weight, and especially at least 8% by weight,based on the total amount of mono-and oligosaccharides in the raw material, of one or more mono- or oligosaccharides different from 2′-FL, where the method comprises a)providing a solution of the 2′-FL raw material in water, which does not contain more than 10% by weight, preferably not more than 7% by weight, more preferably not more than 5% by weight of organic solvents, based on the total amount of water; b) effecting the crystallization of 2′-FL from the solution provided in step a) by inducing conditions of a controlled super saturation in the solution; and c) separating crystalline 2′-FL from the mother liquor, and where during controlled supersaturation in step b) not more than 10% by weight, preferably not more than 7% by weight, more preferably not more than 5% by weight of organic solvents are present, based on the total amount of water present during step b).