In Situ Crystallization Device for Macromolecule Structure Determination

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Solution Overview

Problem

Current crystallization techniques for macromolecules, such as proteins, face challenges in obtaining high-quality single crystals of sufficient volume due to issues like polymorphism, low diffraction power, and the inability to control multiple crystallization parameters simultaneously, which hampers the resolution of three-dimensional structures.

Innovation Solution

A crystallization device that allows for the controlled variation of the composition of the crystallization solution in situ, using a multichannel peristaltic pump and dialysis membrane to adjust the concentration of crystallization agents, additives, and buffers, enabling optimization of nucleation and crystal growth based on phase diagrams, thereby achieving crystals of excellent quality and desired polymorphous phase.

Engineering Contradictions & Design Principles

VSEngineering Contradiction Analysis

1Manufacturing precision

If conventional crystallization techniques are used, then crystal formation occurs, but the quality and volume of crystals are insufficient for high-resolution diffraction studies

Engineering Contradiction:
Improvecrystal qualityVSAvoidcrystal volume
Core Design Contradiction:
Manufacturing precisionVSVolume of moving object

Solution Approach 1:

The invention changes multiple crystallization parameters simultaneously including temperature, pH, and concentration of crystallization agents to optimize both crystal quality and volume. The system monitors and adjusts these parameters in real-time to achieve high-quality crystals of sufficient volume for diffraction studies.

Inventive Principle:
Principle #35Parameter changes

Solution Approach 2:

The invention implements feedback control by monitoring crystal growth in real-time and adjusting crystallization parameters accordingly. This feedback mechanism allows the system to maintain optimal conditions for producing high-quality, large-volume crystals by responding to actual crystal formation progress.

Inventive Principle:
Principle #23Feedback

2Manufacturing precision

If multiple crystallization parameters are adjusted simultaneously, then crystal quality improves, but the complexity of the crystallization process increases

Engineering Contradiction:
Improvecrystal qualityVSAvoidcrystallization process complexity
Core Design Contradiction:
Manufacturing precisionVSDevice complexity

Solution Approach 1:

The invention uses a multi-functional crystallization device that can simultaneously control temperature, pH, and concentration parameters while also monitoring crystal growth. This universal system integrates multiple functions into a single platform, managing complexity through integration rather than separate systems.

Inventive Principle:
Principle #6Universality (Multi-functionality)

Solution Approach 2:

The invention introduces a microfluidic intermediary system that mediates between parameter control mechanisms and crystal growth. This intermediary layer simplifies the overall process by providing a controlled environment where multiple parameters can be adjusted without directly complicating the crystal formation process.

Inventive Principle:
Principle #24Intermediary (Mediator)

3Quantity of substance

If large volumes of sample are used for crystallization, then sufficient crystal material is obtained, but the cost and time of research work increase

Engineering Contradiction:
Improvesample volumeVSAvoidresearch time
Core Design Contradiction:
Quantity of substanceVSLoss of time

Solution Approach 1:

The invention performs preliminary optimization of crystallization conditions using small sample volumes before scaling up. By pre-determining optimal parameters with minimal sample, the system avoids wasting large amounts of time and material on suboptimal crystallization attempts.

Inventive Principle:
Principle #10Preliminary action

Solution Approach 2:

The invention uses dynamic control of crystallization conditions to accelerate the process. By continuously adjusting parameters based on real-time monitoring, the system achieves faster crystal formation compared to static conventional methods, reducing both time and sample consumption.

Inventive Principle:
Principle #15Dynamics

4Stability of the object's composition

If crystallization conditions are optimized for one polymorph, then that specific crystal form is obtained, but the ability to produce other polymorphs is lost

Engineering Contradiction:
Improvepolymorph consistencyVSAvoidpolymorph flexibility
Core Design Contradiction:
Stability of the object's compositionVSAdaptability or versatility

Solution Approach 1:

The invention implements dynamic control of crystallization parameters that can be adjusted to favor different polymorphs. By making the system adaptable rather than fixed, it can transition between producing different crystal forms based on desired outcomes, maintaining both consistency for a given polymorph and flexibility to switch between polymorphs.

Inventive Principle:
Principle #15Dynamics

Applied Scientific Principles

This section explains which scientific principles are used to turn an abstract innovation direction into a practical engineering solution.

Function Achieved in This Case

This approach allows for the production of high-quality, large-volume crystals with controlled polymorphous phase, reducing the amount of sample used and enabling reuse, while minimizing mechanical perturbations and optimizing crystal size and diffraction quality, thus facilitating the determination of macromolecular structures.

Implementation Method 1

using a multichannel peristaltic pump and dialysis membrane to adjust the concentration of crystallization agents, additives, and buffers

Methodology Applied
Scientific EffectDialysis: Semipermeable Membrane

Implementation Method 2

allows for the controlled variation of the composition of the crystallization solution in situ

Methodology Applied
Scientific EffectDiffusion: Diffusion

Data Source

PatentUS9382639B2Device and method for crystallizing inorganic or organic substances
Publication Date: 2016.07.05 UNIVERSITE GRENOBLE ALPES

AI summary

A device for crystallizing a molecule to be crystallized, which includes: at least one crystallization cell that includes a crystallization chamber for receiving a first solution S1 containing the molecule to be crystallized and the crystal seeds thereof, a dialysis membrane, and a container to be filled with a second solution S2 that contains constituents selected from the group containing crystallization agents, additives and buffers; and at least one image acquisition means. The crystallization device is characterized in that it includes: at least one addition means arranged to add, to the container, constituents selected from the group containing crystallization agents, additives and buffers of solution S2; and/or at least one sampling means arranged to collect, from the container, all or a portion of solution S2. The invention also relates to a crystallization method.