Angular Leaf Spot Resistance in Cucumber via Genetic Modification
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Solution Overview
Problem
Cucumber plants are susceptible to angular leaf spot disease caused by Pseudomonas syringae pv. lachrymans, which affects global cucumber production, particularly under humid and warm conditions, leading to significant yield losses and crop damage.
Innovation Solution
Development of cucumber plants with resistance to Pseudomonas syringae pv. lachrymans through the identification and utilization of a mutant allele of the Gibberellin-20-oxidase gene and minor QTLs, such as QTL2, QTL3, QTL4, and QTLS, which confer resistance by altering the plant's genetic makeup to hinder bacterial infection.
Engineering Contradictions & Design Principles
Engineering Contradiction Analysis
1Productivity
If conventional cucumber plants are cultivated under humid and warm conditions, then high productivity can be achieved, but the plants become susceptible to angular leaf spot disease caused by Pseudomonas syringae pv. lachrymans
Solution Approach 1:
The patent applies parameter changes by modifying the genetic parameters of cucumber plants through mutant alleles of the Gibberellin-20-oxidase gene and QTLs. This genetic modification changes the plant's physiological parameters to confer resistance to Pseudomonas syringae pv. lachrymans, allowing the plants to maintain productivity under humid and warm conditions without becoming susceptible to angular leaf spot disease
2Reliability
If cucumber plants are bred for resistance to Pseudomonas syringae pv. lachrymans through mutant alleles and QTLs, then disease resistance is improved, but the complexity of plant development and breeding increases
Solution Approach 1:
The patent applies preliminary action by pre-identifying and characterizing the mutant allele of the Gibberellin-20-oxidase gene and associated QTLs that confer resistance to Pseudomonas syringae pv. lachrymans. Molecular markers are developed in advance to track these resistance traits during breeding, allowing breeders to efficiently select resistant plants without complex phenotypic screening, thus reducing breeding program complexity while maintaining high disease resistance
Solution Approach 2:
The patent uses molecular markers as intermediaries to connect the resistance genes (mutant allele of Gibberellin-20-oxidase and QTLs) with the breeding process. These markers serve as detectable indicators that facilitate the selection of resistant plants, simplifying the breeding program by providing a straightforward method to track and select for the desired resistance traits without requiring complex genetic manipulation
Data Source
AI summary
The present invention relates to a cucumber plant (Cucumis sativus L.) that is resistant to Pseudomonas syringae pv. lachrymans, a pathogen that causes angular leaf spot disease in cucumber. The invention further relates to markers linked to the resistance and the use of markers to identify resistant plants. The invention also relates to seed and progeny of such plants and to propagation material derived from such plants and for obtaining such plants. Furthermore, the invention relates to methods for producing, identifying and selecting Pseudomonas resistant cucumber plants, and for producing hybrid plant seed. The invention further relates to the use of a gene and/or QTLs and sequences thereof to identify/select Pseudomonas resistant cucumber plants.