Cucumber CABYV Resistance Locus Introgression With Marker Selection
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Solution Overview
Problem
There is a lack of effective resistance to cucurbit aphid-borne yellows virus (CABYV) in cucumber plants, which can lead to significant yield loss, and existing methods for resistance, such as chemical control and physical barriers, have limitations.
Innovation Solution
Identifying and introgressing a chromosomal segment flanked by marker loci M1 and M2 on chromosome 4 of the cucumber genome, which confers increased resistance to CABYV, using marker-assisted selection and genetic markers to track and breed plants with this resistance.
Engineering Contradictions & Design Principles
Engineering Contradiction Analysis
1Reliability
If chemical control and physical barriers are used to protect against CABYV, then resistance to the virus is provided, but environmental harm and other limitations occur
Solution Approach 1:
The patent converts the harmful effect of viral infection into a beneficial outcome by identifying and utilizing natural genetic resistance mechanisms. Instead of applying external chemical controls that harm the environment, the invention harnesses the plant's own genetic potential for resistance through marker-assisted selection, thereby eliminating environmental harm while maintaining reliable protection against CABYV
Solution Approach 2:
The patent replaces mechanical and chemical control systems (sprays, barriers) with a biological system based on genetic resistance. By substituting external chemical interventions with internally-derived genetic resistance mechanisms, the invention achieves reliable virus protection without the environmental damage associated with chemical controls
2Manufacturing precision
If marker-assisted selection is used to introgress resistance alleles, then breeding precision is improved, but time and resources for screening are increased
Solution Approach 1:
The patent applies preliminary action by developing and validating marker assays before the breeding process begins. The marker loci are identified and characterized in advance, allowing breeders to immediately use these pre-established tools for rapid selection. This preliminary preparation eliminates the need for time-consuming phenotypic screening during the breeding process, thereby reducing time loss while maintaining high breeding precision
Solution Approach 2:
The patent uses molecular markers as copies or proxies for the actual resistance alleles. Instead of directly observing and selecting for the complex resistance trait (which would be time-consuming), the invention uses easily detectable DNA marker copies that are genetically linked to the resistance alleles. This copying approach allows rapid identification of resistant plants without lengthy phenotypic evaluation, thus reducing time loss while preserving breeding precision
Data Source
AI summary
Cucumber plants exhibiting resistance to cucurbit aphid-borne yellows virus (CABYV) are provided, together with methods of producing, identifying, or selecting plants or germplasm with a cucurbit aphid-borne yellows virus resistance phenotype. Such methods include producing a cucumber plant exhibiting resistance to CABYV, comprising introgressing genomic regions conferring disease resistance; or selecting a cucumber plant exhibiting resistance to CABYV. Compositions, including polymorphic markers for detecting plants comprising introgressed disease resistance alleles, are further provided.