Cucumber Plant FORC Resistance via SNP-Marked Chromosomal Segments
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Solution Overview
Problem
Existing methods for introgressing Fusarium oxysporum f.sp. radicis cucumerinum (FORC) resistance alleles into cucumber plants are hindered by the association with undesirable traits such as necrosis, poor fruit shape, and agronomically unacceptable plant architecture, and lack of accurate markers for tracking these alleles.
Innovation Solution
The introduction of recombinant chromosomal segments flanked by specific SNP markers (e.g., SNP_Marker6 and SNP_Marker7 on chromosome 6, SNP_Marker1 and SNP_Marker2 on chromosome 3) that confer resistance to FORC without deleterious traits, accompanied by novel markers for accurate marker-assisted selection (MAS).
Engineering Contradictions & Design Principles
Engineering Contradiction Analysis
1Reliability
If FORC resistance alleles are introgressed into cucumber plants using conventional breeding methods, then disease resistance is improved, but undesirable traits such as necrosis, poor fruit shape, and unacceptable plant architecture are also introduced
Solution Approach 1:
The patent divides the chromosome into small segments flanked by specific SNP markers (e.g., SNP_Marker6 and SNP_Marker7 on chromosome 6, or SNP_Marker1 and SNP_Marker2 on chromosome 3). By selecting and introgressing only these specific small segments containing the FORC resistance allele, the invention isolates the beneficial resistance trait from the harmful linked traits that would otherwise be co-introgressed in conventional breeding.
Solution Approach 2:
The patent applies local quality by making the chromosomal segment have different properties at different locations: the central region contains the FORC resistance allele with desired properties, while the flanking regions are defined by specific SNP markers that ensure absence of deleterious alleles. This localized differentiation allows the resistance trait to be expressed without the harmful linked traits.
2Measurement precision
If marker-assisted selection is used to track resistance alleles, then selection accuracy is improved, but accurate markers linked to FORC resistance alleles were previously unavailable
Solution Approach 1:
The patent performs preliminary action by identifying and validating specific SNP markers (SNP_Marker1 through SNP_Marker11) that are linked to the FORC resistance allele before the breeding process begins. These markers are pre-characterized and can be used to track the resistance allele through generations, enabling accurate marker-assisted selection from the outset of the breeding program.
Data Source
AI summary
Cucumber plants exhibiting resistance to Fusarium oxysporum f.sp. radicis cucumerinum (FORC) are provided, together with methods of producing, identifying, or selecting plants or germplasm with a FORC resistance phenotype. Such plants include cucumber plants comprising introgressed genomic regions conferring disease resistance. Compositions, including novel polymorphic markers for detecting plants comprising introgressed disease resistance alleles, are further provided.