Cucumis melo Regulatory Elements for Plant Gene Expression
Find Innovative SolutionsGenerate Solutions
Solution Overview
Problem
Current methods in plant genetic engineering lack effective regulatory elements for modulating gene expression in plants, particularly for achieving desired traits like herbicide resistance and pest resistance, due to limited availability of expression-enhancing introns and inefficient gene regulation.
Innovation Solution
Development of novel gene regulatory elements such as promoters, leaders, and introns derived from Cucumis melo, which are operably linked to transcribable polynucleotide molecules, enabling precise control of gene expression in transgenic plants for agronomically beneficial traits.
Engineering Contradictions & Design Principles
Engineering Contradiction Analysis
1Productivity
If conventional regulatory elements are used in plant genetic engineering, then basic gene expression is maintained, but gene expression enhancement and precision control are insufficient
Solution Approach 1:
The patent combines multiple regulatory elements (promoters, introns, leaders) from Cucumis melo into composite regulatory structures. These composite elements work synergistically to enhance gene expression levels while maintaining reliable regulation, resolving the contradiction between productivity and reliability in plant genetic engineering.
Solution Approach 2:
The patent modifies regulatory element parameters by using specific Cucumis melo-derived sequences with optimized characteristics. These parameter changes in promoter strength, intron structure, and leader sequence composition enable enhanced gene expression while preserving regulatory precision and reliability.
2Productivity
If novel Cucumis melo regulatory elements are used, then gene expression enhancement is achieved, but availability of such elements was previously limited
Solution Approach 1:
The patent develops universal Cucumis melo regulatory elements that can function across multiple plant species and application contexts. These elements are designed to be broadly applicable for enhancing expression of various transgenes, including those conferring herbicide resistance and pest resistance, thereby increasing availability and adaptability.
Solution Approach 2:
The patent segments the regulatory function into distinct modular elements (promoters, introns, leaders) that can be independently selected and combined. This segmentation allows researchers to assemble customized regulatory structures for different applications, expanding the availability and versatility of regulatory elements for various genetic engineering goals.
3Reliability
If regulatory elements for agronomic traits are developed, then traits like herbicide resistance and pest resistance are improved, but complexity of regulatory structure increases
Solution Approach 1:
The patent performs preliminary characterization and optimization of Cucumis melo regulatory elements before their application in genetic engineering. By pre-validating promoter strength, intron splicing efficiency, and leader sequence functionality, the patent simplifies subsequent regulatory structure design while ensuring reliable expression of agronomic traits like herbicide and pest resistance.
Data Source
AI summary
The invention provides DNA molecules and constructs, including their nucleotide sequences, useful for modulating gene expression in plants and plant cells. Transgenic plants, plant cells, plant parts, seeds, and commodity products comprising the DNA molecules operably linked to heterologous transcribable polynucleotides are also provided, as are methods of their use.