Vacuum-Packed Culture Bag Structure for Air Bubble Removal
Find Innovative SolutionsGenerate Solutions
Solution Overview
Problem
Existing methods for removing air bubbles from culture bags with minute wells are labor-intensive, require dedicated equipment, and complicate the production process, increasing the risk of contamination, especially in regenerative medicine and cellular pharmaceuticals.
Innovation Solution
A liquid-filled culture bag with a microstructure in the culture portion, housed in an outer packaging portion, featuring a depressurizable space between the culture and outer packaging, and vacuum-packaged to efficiently remove air bubbles without additional labor or equipment.
Engineering Contradictions & Design Principles
Engineering Contradiction Analysis
1Ease of operation
If air bubbles are removed by water pressure spraying using a micropipette, then air bubbles can be removed from open containers, but this method cannot be applied to closed culture bags and is ineffective for small wells
Solution Approach 1:
The culture bag is filled with culture medium before sealing, and air bubbles are removed during the filling process rather than attempting removal after sealing. This preliminary action allows effective air bubble removal while maintaining the closed system integrity.
Solution Approach 2:
The patent utilizes pressure differential created during filling to force air bubbles out of the culture bag through the filling needle. By controlling the filling speed and pressure, air bubbles are effectively removed from both large and small wells without requiring direct mechanical intervention.
2Object-generated harmful factors
If dedicated vacuum equipment is used to remove air bubbles from culture bags, then air bubbles can be removed, but this requires complex equipment and increases contamination risk
Solution Approach 1:
The system removes air bubbles using the filling process itself rather than requiring separate vacuum equipment. The pressure differential generated during medium filling automatically forces air bubbles out through the filling needle, making the air bubble removal function self-contained within the filling operation.
Solution Approach 2:
The patent combines the air bubble removal function with the culture medium filling operation. Both functions are performed simultaneously through the same filling needle, eliminating the need for separate vacuum equipment and reducing overall system complexity.
3Object-generated harmful factors
If air bubble removal operations are performed on-site in production processes, then air bubbles can be removed, but this complicates the production process and increases contamination risk
Solution Approach 1:
Air bubble removal is performed as a preliminary step during the filling process before the culture bag is sealed and placed in production. This eliminates the need for subsequent air bubble removal operations that would complicate the production workflow and increase contamination risk.
Solution Approach 2:
The filling and air bubble removal operations are performed continuously in a single step without interruption. The culture medium is filled while simultaneously forcing air bubbles out, maintaining continuous useful action and preventing production delays.
Applied Scientific Principles
This section explains which scientific principles are used to turn an abstract innovation direction into a practical engineering solution.
Function Achieved in This Case
Efficiently removes air bubbles from culture bags, reducing labor, equipment requirements, and contamination risks, simplifying the production process.
Implementation Method 1
a space capable of being depressurized is present between the outside of the culture portion and the outer packaging portion, and the culture bag is vacuum-packaged
Implementation Method 2
pressure is applied to the culture bag to remove air from the inside of the culture bag
Data Source
AI summary
Provided is a liquid-filled culture bag. The liquid-filled culture bag includes a culture bag. The culture bag has a microstructure formed in at least a part of a culture portion and is housed in an outer packaging portion. A space capable of being depressurized is present between the outside of the culture portion and the outer packaging portion, and the culture bag is vacuum-packaged. It is preferable that a plurality of first protrusions be provided outside the culture portion. In addition, it is preferable that a plurality of second protrusions be provided in a region of the outer packaging portion facing the outside of the culture portion. Furthermore, it is preferable that a spacer portion that forms a space capable of being depressurized be provided between the outside of the culture portion and the outer packaging portion.