Culture Base Tapping Control for Gentle Cell Detachment
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Solution Overview
Problem
Existing cell detachment methods cause damage to cells and are inefficient in replacing culture bases during the cell detachment process, leading to leakage and reduced workability.
Innovation Solution
A cell detachment apparatus with a tapping member that vibrates the culture base using a tapping control unit, which controls the tapping member's position to separate from the culture base during stopping, ensuring effective detachment without damage.
Engineering Contradictions & Design Principles
Engineering Contradiction Analysis
1Reliability
If the tapping member stops at a position in contact with the culture base, then the cell detachment function is maintained, but the replacement of the culture base becomes difficult and leakage occurs
Solution Approach 1:
The tapping member is designed to dynamically change its stop position based on operational phase: during cell detachment, it stops at the contact position to maintain detachment function; during culture base replacement, it stops at a separated position to enable easy replacement and prevent leakage. This dynamic adaptability resolves the contradiction between maintaining detachment function and enabling easy replacement.
Solution Approach 2:
The control unit changes the stop position parameter of the tapping member according to the operational phase. By adjusting this positional parameter, the system achieves both reliable cell detachment (when contact position is used) and easy culture base replacement (when separated position is used), eliminating the need for manual intervention and preventing leakage.
2Productivity
If the tapping member continuously contacts the culture base, then cell detachment efficiency is maintained, but interference with culture base replacement occurs
Solution Approach 1:
The tapping member performs periodic tapping actions during cell detachment to maintain high detachment efficiency. Between these periodic detachment operations, the member moves to a separated position, allowing rapid culture base replacement without continuous contact. This periodic operation pattern maintains productivity while minimizing time loss during replacement.
Solution Approach 2:
Before culture base replacement is needed, the tapping member is moved to a predetermined separated position in advance. This preliminary action ensures that when replacement is required, no time is lost moving the member away, thus maintaining high detachment efficiency during operation while enabling rapid replacement when needed.
3Force
If the tapping member stops in contact with the culture base, then detachment force is applied, but leakage and interference occur during replacement
Solution Approach 1:
The control unit acts as an intermediary that manages the stop position of the tapping member based on operational context. During cell detachment, it mediates by positioning the member for effective force application; during replacement, it mediates by positioning the member to prevent leakage and interference, thus eliminating harmful factors while preserving necessary force application.
Applied Scientific Principles
This section explains which scientific principles are used to turn an abstract innovation direction into a practical engineering solution.
Function Achieved in This Case
Improves cell detachment performance by reducing interference and leakage, enhancing workability during culture base replacement, and maintaining high detachment efficiency.
Implementation Method 1
a cell detachment apparatus that releases cells adhering to a culture surface of a culture base, from the culture surface, by tapping the culture base
Data Source
AI summary
A cell detachment apparatus that releases cells adhering to a culture surface of a culture base, from the culture surface, by tapping the culture base, the apparatus includes a tapping member configured to tap the culture base, and a tapping control unit configured to move the tapping member and control a position of the tapping member, wherein the tapping control unit stops the tapping member at a position separated from the culture base.