Multilayer Culture Vessel Buffer Layout for Uniform Cell Peeling
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Solution Overview
Problem
Conventional multilayer culture vessels experience variations in the time cells are immersed in peeling liquid during the peeling process, leading to potential cell damage or incomplete peeling due to uneven distribution of the peeling liquid.
Innovation Solution
A multilayer culture vessel design with a buffer space and communication portions allows for even distribution of peeling liquid by rearranging the vessel to direct the liquid into culture layers through a buffer space, minimizing time variance and ensuring uniform immersion.
Engineering Contradictions & Design Principles
Engineering Contradiction Analysis
1Quantity of substance
If a conventional multilayer culture vessel with a cylindrical liquid supply/drainage part is used, then the vessel can supply liquid to multiple culture layers, but the peeling liquid is not evenly distributed across all culture layers leading to variations in immersion time
Solution Approach 1:
The vessel is segmented into multiple culture layers with intermediate plates, and the liquid supply system is segmented into multiple communication portions distributed across different levels. This segmentation allows peeling liquid to be delivered to each culture layer simultaneously and uniformly, resolving the uneven distribution problem while maintaining the multi-layer culture capability.
Solution Approach 2:
The invention transitions from a single central liquid supply path (cylindrical drainage part) to a multi-dimensional distribution system with communication portions formed at multiple positions on intermediate plates. This dimensional expansion of the liquid supply architecture enables uniform peeling liquid distribution across all culture layers, eliminating immersion time variations.
2Adaptability or versatility
If a cylindrical liquid supply/drainage part penetrates intermediate plates is used, then liquid can be supplied to multiple culture layers, but the device structure becomes complex
Solution Approach 1:
The invention extracts the liquid supply function from the complex cylindrical drainage part structure and implements it through simpler communication portions formed directly on the intermediate plates. This extraction simplifies the overall device structure while preserving the essential liquid supply capability to multiple culture layers.
Solution Approach 2:
The intermediate plates serve multiple functions: they divide the culture space into separate layers, provide structural support, and incorporate communication portions for liquid supply. This multi-functionality eliminates the need for separate complex liquid supply mechanisms, reducing device complexity while maintaining versatility.
Applied Scientific Principles
This section explains which scientific principles are used to turn an abstract innovation direction into a practical engineering solution.
Function Achieved in This Case
The design suppresses variations in immersion time, reduces cell damage, and ensures complete peeling by evenly distributing the peeling liquid across all culture layers without waste.
Implementation Method 1
the peeling liquid flows into the respective culture layers substantially evenly via the communication portions
Data Source
AI summary
A multilayer culture vessel includes: an internal space that is divided by a boundary portion into a culture space on one side and a buffer space on the other side in a direction parallel to a bottom plate; at least one intermediate plate extending along the direction parallel to the bottom plate at least in the culture space and configured to divide the culture space into culture layers; wall portions at the boundary portion, the wall portions including one extending from the bottom plate in a direction toward a top plate and one extending from each of the at least one intermediate plate in a direction toward the top plate; communication portions formed at the boundary portion to bring the culture layers into communication with the buffer space; and a liquid supply/drainage port formed in the housing at a location facing the buffer space.