CuTi Metal Oxide Particles for Selective Nucleic Acid Purification
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Solution Overview
Problem
Existing nucleic acid purification methods are time-consuming and often use hazardous organic solvents, posing risks to operators and requiring multiple extractions.
Innovation Solution
The use of metal or metal oxide compositions, such as CuTi particles, to capture and purify DNA and RNA from biological samples, allowing for efficient binding, washing, and elution of nucleic acids.
Engineering Contradictions & Design Principles
Engineering Contradiction Analysis
1Reliability
If organic extraction procedures are used to purify DNA, then purification effectiveness is improved, but time consumption increases and safety risks increase
Solution Approach 1:
The patent replaces the mechanical/chemical organic extraction system with a magnetic field-based separation system. Magnetic particles coated with nucleic acid-binding molecules are used instead of organic solvents, allowing nucleic acids to be captured through magnetic field application and washed free from contaminants through simple fluid flow, dramatically reducing both time and safety concerns
Solution Approach 2:
The patent changes the fundamental parameter of the purification mechanism from chemical solubility-based extraction to magnetic field-based separation. By coating magnetic particles with nucleic acid-binding molecules and applying a magnetic field, the system achieves rapid separation without requiring multiple extraction cycles or hazardous organic solvents
2Reliability
If organic solvents are used in nucleic acid purification, then purification capability is improved, but safety and operational convenience deteriorate
Solution Approach 1:
The patent substitutes hazardous organic solvent-based chemistry with a physical magnetic field separation system. Magnetic particles with nucleic acid-binding coatings allow for safe, convenient operation through simple magnetic field application and fluid washing, eliminating the need to handle toxic organic chemicals while maintaining effective purification
Solution Approach 2:
The patent introduces magnetic particles as an intermediary medium between the sample and the purification process. These particles bind to nucleic acids through coated molecules and can be easily separated from contaminants using magnetic fields and wash solutions, providing a safe and convenient alternative to direct organic solvent extraction
3Reliability
If multiple extraction steps are performed, then purification completeness is improved, but productivity decreases
Solution Approach 1:
The patent segments the purification function into distinct functional components: magnetic particles with specific binding coatings handle nucleic acid capture, wash solutions handle contamin removal, and magnetic field application handles separation. This modular approach achieves complete purification in a single integrated step rather than requiring multiple sequential extractions
Solution Approach 2:
The magnetic particle system performs multiple functions simultaneously: capturing nucleic acids, separating them from contaminants, and enabling rapid recovery through magnetic field application. This multi-functional approach consolidates what would traditionally require multiple separate extraction steps into a single efficient process
Applied Scientific Principles
This section explains which scientific principles are used to turn an abstract innovation direction into a practical engineering solution.
Function Achieved in This Case
This method provides a rapid, efficient, and safe means of isolating nucleic acids, with CuTi particles selectively capturing RNA while minimizing DNA binding, which is crucial for accurate viral particle quantification.
Implementation Method 1
CuTi particles selectively capturing RNA while minimizing DNA binding
Implementation Method 2
The magnetic particles can be caused to aggregate by the application of a magnetic field
Implementation Method 3
eluting the DNA and/or RNA from the particle or solid support
Data Source
AI summary
The present disclosure relates to systems and methods for purifying nucleic acid. In particular, the present disclosure relates to systems and methods for purifying nucleic acids using metal or metal oxide compositions.