Cutin Extraction with Pressurized Water to Preserve Native Structure

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Solution Overview

Problem

Current methods for isolating cutin from plant material involve harsh processes like alkaline hydrolysis and thermal treatments that depolymerize the native cutin structure, making it unsuitable for industrial-scale applications that require preservation of the native polyester scaffold.

Innovation Solution

A process using high temperatures (300-500 °C) and pressures (85-400 bar) for short reaction times (less than 50 seconds) to extract a cutin-enriched product, preserving the native structure by avoiding depolymerization and efficiently removing non-cutin species.

Engineering Contradictions & Design Principles

VSEngineering Contradiction Analysis

1Productivity

If alkaline hydrolysis or thermal treatment is used to extract cutin, then extraction efficiency is improved, but the native cutin structure is depolymerized and destroyed

Engineering Contradiction:
Improveextraction efficiencyVSAvoidnative cutin structure
Core Design Contradiction:
ProductivityVSStability of the object's composition

Solution Approach 1:

The invention changes the extraction parameters by using subcritical water conditions (temperature 100-300°C, pressure corresponding to saturated vapor pressure) instead of conventional alkaline hydrolysis or high-temperature thermal treatment. This parameter change allows efficient extraction while preserving the native cutin polyester structure, as the milder conditions prevent depolymerization of ester bonds.

Inventive Principle:
Principle #35Parameter changes

Solution Approach 2:

The invention uses water as an intermediary extracting agent under subcritical conditions. Water acts as a mediator that can effectively extract cutin from plant material without causing the harsh chemical degradation associated with alkaline solutions or the thermal depolymerization associated with high-temperature treatments. The subcritical water medium provides selective extraction while maintaining structural integrity.

Inventive Principle:
Principle #24Intermediary (Mediator)

2Quantity of substance

If conventional extraction methods are used, then cutin can be obtained, but the process is complex and requires multiple steps including dewaxing, enzyme treatment, and neutralization

Engineering Contradiction:
Improvecutin productVSAvoidprocess steps
Core Design Contradiction:
Quantity of substanceVSDevice complexity

Solution Approach 1:

The invention extracts cutin directly from plant material using subcritical water treatment, taking out the desired product in a single primary extraction step. This eliminates the need for separate dewaxing steps, enzyme treatment steps, and neutralization steps required by conventional methods, significantly simplifying the overall process while maintaining effective cutin recovery.

Inventive Principle:
Principle #2Taking out (Extraction)

Solution Approach 2:

The invention combines multiple conventional extraction steps into a single integrated subcritical water treatment process. The extraction conditions simultaneously achieve what previously required separate dewaxing, hydrolysis, and purification steps, reducing process complexity from multiple sequential operations to one unified treatment.

Inventive Principle:
Principle #5Merging (Combining)

3Quantity of substance

If alkaline hydrolysis is used, then cutin extraction is achieved, but organic co-solvents and additional separation steps are required

Engineering Contradiction:
Improvecutin productVSAvoidprocess simplicity
Core Design Contradiction:
Quantity of substanceVSEase of manufacture

Solution Approach 1:

The invention uses water as a simple, inexpensive, and environmentally benign extracting agent instead of organic co-solvents. Water can be easily removed and does not require complex recovery systems, making the process simpler and more sustainable. The subcritical water conditions provide sufficient extracting power without the need for additional organic solvents that would complicate the manufacturing process.

Inventive Principle:
Principle #27Cheap short-living objects (Disposable)

4Quantity of substance

If conventional extraction procedures are used, then cutin can be isolated, but the reaction time is long and productivity is low

Engineering Contradiction:
Improvecutin productVSAvoidreaction time
Core Design Contradiction:
Quantity of substanceVSLoss of time

Solution Approach 1:

The invention utilizes the phase transition properties of water under subcritical conditions (temperature 100-300°C, pressure at saturated vapor pressure) to enhance extraction efficiency. The subcritical state of water provides superior solvent properties that accelerate the extraction kinetics, reducing the required reaction time from hours in conventional methods to much shorter durations while maintaining or improving cutin recovery.

Inventive Principle:
Principle #36Phase transitions

Applied Scientific Principles

This section explains which scientific principles are used to turn an abstract innovation direction into a practical engineering solution.

Function Achieved in This Case

The process efficiently enriches cutin while maintaining its native structure, producing a product with high cutin content and suitable for industrial applications in polymers, coatings, cosmetics, and food emulsifiers.

Implementation Method 1

treating said cutin-containing portion with water at high temperatures (i.e. between 300 and 500 °C) and pressures (i.e. between 85 and 400 bar)

Methodology Applied
Scientific EffectSupercritical fluid extraction: Supercritical Fluid Extraction

Data Source

PatentEP4647481A1Process for the obtention of a cutin-enriched product
Publication Date: 2025.11.12 UNIVERSITY OF VALLADOLID
  • EP4647481A1 patent drawingFigure 1~2C
  • EP4647481A1 patent drawingFigure 3A~3C
  • EP4647481A1 patent drawingFigure 4A~5

AI summary

The present invention discloses a process for obtaining a cutin-enriched product from the cutin-containing portion of a plant matter, wherein said process comprises treating said cutin-containing portion with water at high temperatures and pressures. The invention also discloses a cutin-enriched product and uses thereof.