Corn Variety CV045443 Breeding for Genetic Uniformity

Solution Overview

Problem

Corn breeding aims to combine desirable traits like yield, disease resistance, and uniformity, but existing methods struggle to develop stable inbred plants with predictable performance due to genetic non-uniformity from cross-pollination, leading to unpredictable hybrid traits.

Innovation Solution

The development of the corn variety CV045443, which includes a cytoplasmic or nuclear factor for male sterility to prevent self-pollination, along with specific genetic loci for traits like herbicide tolerance and disease resistance, and the use of tissue cultures to regenerate plants with consistent morphological and physiological characteristics.

Engineering Contradictions & Design Principles

VSEngineering Contradiction Analysis

1Adaptability or versatility

If cross-pollination is used to combine desirable traits from different plants, then genetic diversity and trait combination are improved, but genetic uniformity and predictability of hybrid performance deteriorate

Engineering Contradiction:
Core Design Contradiction:
Adaptability or versatilityVSStability of the object's composition

Solution Approach 1:

The breeding process is segmented into distinct phases: first developing homozygous inbred lines through self-pollination to achieve genetic uniformity, then crossing these stabilized lines to combine desirable traits. This segmentation allows each phase to optimize for its specific goal without compromising the other.

Inventive Principle:
Principle #1Segmentation

Solution Approach 2:

Homozygous inbred plants are developed in advance through multiple generations of self-pollination and selection before the cross-pollination step. This preliminary stabilization of genetic composition ensures that when crossing occurs, the resulting hybrids have predictable performance based on the known parental lines.

Inventive Principle:
Principle #10Preliminary action

2Stability of the object's composition

If self-pollination is used to develop uniform inbred plants, then genetic uniformity and stability are improved, but genetic diversity and ability to combine traits deteriorate

Engineering Contradiction:
Core Design Contradiction:
Stability of the object's compositionVSAdaptability or versatility

Solution Approach 1:

Self-pollination and selection are performed in advance to develop homozygous inbred lines with stable, uniform genetics. Once these parental lines are established with desired traits, cross-pollination is then used to combine them, achieving both uniformity within lines and diversity in trait combination.

Inventive Principle:
Principle #10Preliminary action

Solution Approach 2:

The breeding program is divided into separate self-pollination phases for developing individual inbred lines, followed by cross-pollination phases for combining them. This segmentation allows self-pollination to achieve uniformity while cross-pollination provides trait combination, with each phase optimized for its specific function.

Inventive Principle:
Principle #1Segmentation

3Stability of the object's composition

If traditional breeding methods are used to develop inbred plants, then genetic stability is improved, but breeding time and complexity increase

Engineering Contradiction:
Core Design Contradiction:
Stability of the object's compositionVSLoss of time

Solution Approach 1:

Molecular marker technology replaces traditional phenotypic selection methods. DNA markers allow breeders to track and select for desired genetic traits directly at the molecular level, eliminating the need for lengthy field testing and phenotypic evaluation across multiple generations, thus reducing breeding time while maintaining genetic stability.

Inventive Principle:
Principle #28Mechanics substitution (Replace mechanical system)

4Stability of the object's composition

If male sterility factors are introduced to prevent self-pollination, then cross-pollination control and hybrid uniformity are improved, but genetic manipulation complexity increases

Engineering Contradiction:
Core Design Contradiction:
Stability of the object's compositionVSDevice complexity

Solution Approach 1:

Male sterility systems enable the female parent to self-regulate pollination control without requiring manual emasculation or complex mechanical interventions. The sterile parent automatically prevents self-pollination, ensuring cross-pollination occurs only with the desired male parent, thereby maintaining hybrid uniformity while simplifying the breeding operation.

Inventive Principle:
Principle #25Self-service

Data Source

PatentUS9485937B2Plants and seeds of corn variety CV045443
Publication Date: 2016.11.08

AI summary

According to the invention, there is provided seed and plants of the corn variety designated CV045443. The invention thus relates to the plants, seeds and tissue cultures of the variety CV045443, and to methods for producing a corn plant produced by crossing a corn plant of variety CV045443 with itself or with another corn plant, such as a plant of another variety. The invention further relates to corn seeds and plants produced by crossing plants of variety CV045443 with plants of another variety, such as another inbred line. The invention further relates to the inbred and hybrid genetic complements of plants of variety CV045443.