Corn Variety CV099177 Breeding for Genetic Uniformity

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Solution Overview

Problem

Corn breeding aims to combine desirable traits like yield, disease resistance, and uniformity, but existing methods struggle to develop stable inbred plants with predictable performance due to genetic non-uniformity from cross-pollination, leading to unpredictable hybrid traits.

Innovation Solution

The development of the corn variety CV099177, which includes a cytoplasmic or nuclear factor for male sterility and specific genetic loci for traits like herbicide tolerance and disease resistance, along with a process for producing inbred and hybrid seeds through controlled pollination and backcrossing techniques, ensures genetic stability and uniformity.

Engineering Contradictions & Design Principles

VSEngineering Contradiction Analysis

1Adaptability or versatility

If cross-pollination is used in corn breeding, then genetic diversity is increased, but genetic uniformity and predictability of hybrid traits deteriorate

Engineering Contradiction:
Improvegenetic diversityVSAvoidgenetic uniformity
Core Design Contradiction:
Adaptability or versatilityVSStability of the object's composition

Solution Approach 1:

The breeding process is segmented into distinct phases: first developing homozygous inbred lines through self-pollination to achieve genetic uniformity, then crossing these stabilized lines to introduce controlled genetic diversity. This segmentation allows each phase to optimize for its specific goal without compromising the other.

Inventive Principle:
Principle #1Segmentation

Solution Approach 2:

Homozygous inbred lines are developed in advance through multiple generations of self-pollination and selection before the actual hybrid crossing. This preliminary stabilization of parental lines ensures that when cross-pollination occurs, the genetic diversity introduced is predictable and based on known parental genotypes.

Inventive Principle:
Principle #10Preliminary action

2Stability of the object's composition

If self-pollination is used to develop inbred lines, then genetic uniformity is improved, but breeding efficiency and time to develop diverse hybrids deteriorate

Engineering Contradiction:
Improvegenetic uniformityVSAvoidbreeding efficiency
Core Design Contradiction:
Stability of the object's compositionVSProductivity

Solution Approach 1:

The self-pollination process is continued uninterrupted over multiple generations (typically 6-10 generations) to ensure complete homozygosity. This continuous application of selfing without interruption ensures that genetic uniformity is fully achieved, creating stable parental lines that will produce predictable hybrids.

Inventive Principle:
Principle #20Continuity of useful action

Solution Approach 2:

Once stable inbred lines are developed, they serve as reusable parental copies that can be crossed with other standardized inbreds. These 'genetic templates' can be propagated and used repeatedly in different crossing combinations, increasing overall breeding efficiency without sacrificing the uniformity achieved through selfing.

Inventive Principle:
Principle #26Copying

3Stability of the object's composition

If multiple breeding generations are conducted to achieve homozygosity, then genetic stability is improved, but time to market and development duration deteriorate

Engineering Contradiction:
Improvegenetic stabilityVSAvoiddevelopment duration
Core Design Contradiction:
Stability of the object's compositionVSLoss of time

Solution Approach 1:

Molecular marker technology and DNA-based genotyping methods replace traditional phenotypic selection and lengthy multi-generation testing. These molecular tools allow breeders to assess genetic stability and homozygosity directly at the DNA level, dramatically reducing the time required to confirm genetic stability without compromising accuracy.

Inventive Principle:
Principle #28Mechanics substitution (Replace mechanical system)

Solution Approach 2:

The breeding program uses accelerated generation techniques and optimizes growth conditions to compress the developmental timeline. By changing environmental parameters (temperature, photoperiod, nutrient supply) and using tissue culture methods, multiple generations can be produced in reduced time while maintaining the genetic stability that would normally require extended breeding cycles.

Inventive Principle:
Principle #35Parameter changes

Data Source

PatentUS9179636B2Plants and seeds of corn variety CV099177
Publication Date: 2015.11.10 MONSANTO TECHNOLOGY LLC

AI summary

According to the invention, there is provided seed and plants of the corn variety designated CV099177. The invention thus relates to the plants, seeds and tissue cultures of the variety CV099177, and to methods for producing a corn plant produced by crossing a corn plant of variety CV099177 with itself or with another corn plant, such as a plant of another variety. The invention further relates to corn seeds and plants produced by crossing plants of variety CV099177 with plants of another variety, such as another inbred line. The invention further relates to the inbred and hybrid genetic complements of plants of variety CV099177.