Corn Variety CV194481 Breeding via Segmentation and Markers

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Solution Overview

Problem

Current corn breeding techniques face challenges in developing uniform corn hybrids with desirable traits such as high yield, disease resistance, and uniform germination due to the unpredictability of genetic non-uniformity in cross-pollinated plants, which requires the development of stable inbred plants and precise breeding methods.

Innovation Solution

The development of the corn variety CV194481, which includes a cytoplasmic or nuclear factor for male sterility to prevent self-pollination, along with specific genetic loci for traits like herbicide tolerance, insect resistance, and modified metabolism, and the use of tissue cultures to regenerate plants with consistent physiological and morphological characteristics.

Engineering Contradictions & Design Principles

VSEngineering Contradiction Analysis

1Adaptability or versatility

If cross-pollination is used in corn breeding, then genetic diversity and adaptability are improved, but genetic uniformity and predictability of performance deteriorate

Engineering Contradiction:
Improvegenetic diversityVSAvoidgenetic uniformity
Core Design Contradiction:
Adaptability or versatilityVSStability of the object's composition

Solution Approach 1:

The breeding process is segmented into distinct phases: first developing homozygous inbred lines through self-pollination to ensure genetic uniformity, then crossing these standardized lines to introduce controlled genetic diversity. This segmentation allows each phase to optimize for its specific goal without compromising the other.

Inventive Principle:
Principle #1Segmentation

Solution Approach 2:

Homozygous inbred plants are developed in advance through multiple generations of self-pollination and selection before the crossing stage. This preliminary action ensures that the parental lines have stable, uniform genetics, which then allows the F1 hybrid generation to exhibit consistent and predictable performance.

Inventive Principle:
Principle #10Preliminary action

2Stability of the object's composition

If self-pollination is used to develop homozygous inbred plants, then genetic uniformity and true breeding are improved, but time to achieve homozygosity and breeding cycles worsen

Engineering Contradiction:
ImprovehomozygosityVSAvoidbreeding cycle time
Core Design Contradiction:
Stability of the object's compositionVSLoss of time

Solution Approach 1:

Molecular marker technology replaces traditional phenotypic selection methods. Instead of visually assessing plant traits over multiple generations to confirm homozygosity, breeders can directly genotype plants to identify those that have achieved the desired homozygous state, dramatically reducing the time required to develop inbred lines.

Inventive Principle:
Principle #28Mechanics substitution (Replace mechanical system)

Solution Approach 2:

The breeding program utilizes controlled environmental conditions and standardized management practices to accelerate development while maintaining homozygosity. By optimizing growth parameters such as temperature, light, and nutrition, the breeder can speed up the inbreeding process without compromising genetic uniformity.

Inventive Principle:
Principle #35Parameter changes

3Reliability

If multiple desirable traits are combined in a single variety, then overall crop performance and resistance are improved, but breeding complexity and difficulty of selection worsen

Engineering Contradiction:
Improvedisease resistanceVSAvoidbreeding program complexity
Core Design Contradiction:
ReliabilityVSDevice complexity

Solution Approach 1:

Molecular markers serve as intermediaries between the breeder and the target traits. Instead of directly selecting for complex traits like disease resistance and yield simultaneously, breeders use markers linked to these traits to identify plants carrying the desired gene combinations, simplifying the selection process while maintaining reliability.

Inventive Principle:
Principle #24Intermediary (Mediator)

Solution Approach 2:

The breeding program employs a unified approach using molecular marker-assisted selection across multiple traits and stages. The same marker technology and selection framework are applied whether selecting for disease resistance, yield components, or other desirable traits, reducing overall breeding complexity through methodological universality.

Inventive Principle:
Principle #6Universality (Multi-functionality)

4Stability of the object's composition

If inbred plants are crossed to produce uniform hybrid populations, then performance predictability and uniformity are improved, but requirement for homozygous parental lines and breeding steps worsen

Engineering Contradiction:
Improvehybrid uniformityVSAvoidbreeding method complexity
Core Design Contradiction:
Stability of the object's compositionVSDevice complexity

Solution Approach 1:

Molecular marker technology replaces complex phenotypic tracking and verification methods. By using markers to confirm homozygosity in parental lines and to track inheritance in hybrid progeny, the breeder ensures uniformity with greater efficiency and reduced complexity compared to traditional visual selection methods.

Inventive Principle:
Principle #28Mechanics substitution (Replace mechanical system)

Data Source

PatentUS7709712B1Plants and seeds of corn variety CV194481
Publication Date: 2010.05.04 MONSANTO TECHNOLOGY LLC

AI summary

According to the invention, there is provided seed and plants of the corn variety designated CV194481. The invention thus relates to the plants, seeds and tissue cultures of the variety CV194481, and to methods for producing a corn plant produced by crossing a corn plant of variety CV194481 with itself or with another corn plant, such as a plant of another variety. The invention further relates to corn seeds and plants produced by crossing plants of variety CV194481 with plants of another variety, such as another inbred line. The invention further relates to the inbred and hybrid genetic complements of plants of variety CV194481.