Corn Variety CV332045 Breeding for Genetic Uniformity

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Solution Overview

Problem

Corn breeding aims to combine desirable traits like yield, disease resistance, and uniformity, but existing methods often result in unpredictable performance due to genetic non-uniformity in hybrid plants, necessitating the development of stable inbred plants with specific characteristics.

Innovation Solution

The development of the corn variety CV332045, which includes a cytoplasmic or nuclear factor for male sterility and additional heritable traits such as herbicide tolerance and disease resistance, achieved through backcrossing and genetic transformation, ensuring uniformity and desirable characteristics in progeny plants.

Engineering Contradictions & Design Principles

VSEngineering Contradiction Analysis

1Adaptability or versatility

If cross-pollination is used to combine desirable traits from different plants, then genetic diversity and trait combination are improved, but genetic non-uniformity and unpredictable performance occur

Engineering Contradiction:
Improvetrait combinationVSAvoidperformance predictability
Core Design Contradiction:
Adaptability or versatilityVSReliability

Solution Approach 1:

The breeding process is segmented into distinct phases: creating homozygous inbred lines through multiple generations of self-pollination, then using these stable lines as parents for hybrid production. This segmentation allows each phase to be optimized independently - first for genetic uniformity, then for trait combination.

Inventive Principle:
Principle #1Segmentation

Solution Approach 2:

Homozygous inbred plants are developed through preliminary self-pollination and selection over multiple generations before the actual hybrid crossing occurs. This preliminary action ensures that the parent lines are genetically stable and uniform, which then enables predictable and reliable hybrid performance.

Inventive Principle:
Principle #10Preliminary action

2Stability of the object's composition

If self-pollination is used to develop homozygous inbred plants, then genetic uniformity is improved, but loss of genetic diversity occurs

Engineering Contradiction:
Improvegenetic uniformityVSAvoidgenetic diversity
Core Design Contradiction:
Stability of the object's compositionVSAdaptability or versatility

Solution Approach 1:

Multiple generations of self-pollination and selection are performed as a preliminary action to achieve homozygosity and genetic uniformity in the inbred lines. This preliminary process creates stable genetic backgrounds that can then be combined in controlled crosses to generate diversity when needed.

Inventive Principle:
Principle #10Preliminary action

Solution Approach 2:

The breeding program is segmented into separate functions: inbred line development (using self-pollination for uniformity) and hybrid production (using cross-pollination for diversity). This segmentation allows each function to be optimized without compromising the other.

Inventive Principle:
Principle #1Segmentation

3Stability of the object's composition

If multiple generations of selfing and selection are performed to develop inbreds, then homozygosity is improved, but time consumption increases

Engineering Contradiction:
ImprovehomozygosityVSAvoidbreeding cycle duration
Core Design Contradiction:
Stability of the object's compositionVSLoss of time

Solution Approach 1:

The development of homozygous inbred plants is performed as a preliminary action that is completed once and reused across multiple breeding cycles. Although time-consuming initially, the resulting stable inbred lines can be crossed repeatedly without requiring re-development, thereby reducing overall time consumption.

Inventive Principle:
Principle #10Preliminary action

Solution Approach 2:

Once a homozygous inbred line is developed, it can be replicated and reused as a parent line in multiple hybrid production cycles. This copying of the stable genetic background eliminates the need to re-create the inbred line each time, significantly reducing time loss in subsequent breeding operations.

Inventive Principle:
Principle #26Copying

Data Source

PatentUS8013224B2Plants and seeds of corn variety CV332045
Publication Date: 2011.09.06 MONSANTO TECHNOLOGY LLC

AI summary

According to the invention, there is provided seed and plants of the corn variety designated CV332045. The invention thus relates to the plants, seeds and tissue cultures of the variety CV332045, and to methods for producing a corn plant produced by crossing a corn plant of variety CV332045 with itself or with another corn plant, such as a plant of another variety. The invention further relates to corn seeds and plants produced by crossing plants of variety CV332045 with plants of another variety, such as another inbred line. The invention further relates to the inbred and hybrid genetic complements of plants of variety CV332045.