Corn Variety CV356454 Genetic Uniformity Breeding

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Solution Overview

Problem

Corn breeding aims to combine desirable traits like yield, disease resistance, and uniformity, but existing methods struggle to develop stable inbred plants with predictable performance due to genetic non-uniformity from cross-pollination, leading to unpredictable hybrid traits.

Innovation Solution

The development of the corn variety CV356454, which includes a cytoplasmic or nuclear factor for male sterility and specific genetic loci conferring traits like herbicide tolerance and disease resistance, along with tissue culture methods for regenerating plants with consistent physiological and morphological characteristics.

Engineering Contradictions & Design Principles

VSEngineering Contradiction Analysis

1Adaptability or versatility

If cross-pollination is used in corn breeding, then genetic diversity is increased, but genetic uniformity and predictability of hybrid traits deteriorate

Engineering Contradiction:
Improvegenetic diversityVSAvoidgenetic uniformity
Core Design Contradiction:
Adaptability or versatilityVSStability of the object's composition

Solution Approach 1:

The breeding program segments the breeding population into distinct inbred lines through repeated self-pollination, creating genetically uniform groups. This segmentation allows controlled crossing between specific segments (inbreds) to produce uniform hybrids with predictable traits, resolving the contradiction between genetic diversity and uniformity.

Inventive Principle:
Principle #1Segmentation

Solution Approach 2:

The patent applies preliminary action by developing homozygous inbred lines through multiple generations of self-pollination before performing the actual hybrid cross. This preliminary homozygization ensures that when crossing occurs, the resulting hybrids will have uniform and predictable expression of traits, eliminating the genetic non-uniformity problem.

Inventive Principle:
Principle #10Preliminary action

2Stability of the object's composition

If self-pollination is used to develop inbred plants, then genetic uniformity is improved, but breeding efficiency and time to develop new varieties deteriorate

Engineering Contradiction:
Improvegenetic uniformityVSAvoidbreeding efficiency
Core Design Contradiction:
Stability of the object's compositionVSProductivity

Solution Approach 1:

The breeding program uses periodic action by systematically rotating through multiple generations of self-pollination (typically 6-8 generations) to achieve homozygosity. This periodic repetition of selfing cycles efficiently produces uniform inbred lines while maintaining organized tracking of genetic progress, balancing uniformity achievement with breeding efficiency.

Inventive Principle:
Principle #19Periodic action

Solution Approach 2:

The patent employs copying by creating multiple replicate lines from the same parental genotype through controlled self-pollination. These replicated lines serve as genetic copies that can be evaluated in parallel, accelerating the identification of superior genotypes and improving overall breeding efficiency while maintaining genetic uniformity within each line.

Inventive Principle:
Principle #26Copying

3Manufacturing precision

If multiple generations of selfing are performed to achieve homozygosity, then uniformity of inbred plants is improved, but time and resources required for breeding deteriorate

Engineering Contradiction:
Improveuniformity of inbred plantsVSAvoidtime to maturity
Core Design Contradiction:
Manufacturing precisionVSLoss of time

Solution Approach 1:

The breeding program applies self-service by allowing the plants to self-pollinate naturally over multiple generations without intensive human intervention. This self-service approach to achieving homozygosity reduces labor and resource requirements compared to manual emasculation and controlled crossing, while still producing uniform inbred lines through the natural selfing process repeated over generations.

Inventive Principle:
Principle #25Self-service

Solution Approach 2:

The patent uses parameter changes by monitoring and selecting for specific phenotypic parameters (such as plant height, ear position, and disease resistance) during the selfing process. By changing selection parameters across generations and using molecular markers to track homozygosity, the program accelerates the achievement of uniformity while reducing the number of generations required compared to traditional methods.

Inventive Principle:
Principle #35Parameter changes

Data Source

PatentUS9210885B2Plants and seeds of corn variety CV356454
Publication Date: 2015.12.15 MONSANTO TECHNOLOGY LLC

AI summary

According to the invention, there is provided seed and plants of the corn variety designated CV356454. The invention thus relates to the plants, seeds and tissue cultures of the variety CV356454, and to methods for producing a corn plant produced by crossing a corn plant of variety CV356454 with itself or with another corn plant, such as a plant of another variety. The invention further relates to corn seeds and plants produced by crossing plants of variety CV356454 with plants of another variety, such as another inbred line. The invention further relates to the inbred and hybrid genetic complements of plants of variety CV356454.