Corn Variety CV382815 Breeding for Stable Inbred Lines

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Solution Overview

Problem

Corn breeding aims to combine desirable traits like yield, disease resistance, and uniformity, but existing methods struggle to produce stable inbred plants with predictable performance due to genetic non-uniformity from cross-pollination, leading to unpredictable hybrid traits.

Innovation Solution

The development of the corn variety CV382815, which includes a cytoplasmic or nuclear factor for male sterility to prevent self-pollination, along with specific genetic loci for traits like herbicide tolerance and disease resistance, and the use of tissue cultures to regenerate plants with consistent physiological and morphological characteristics.

Engineering Contradictions & Design Principles

VSEngineering Contradiction Analysis

1Adaptability or versatility

If cross-pollination is used to combine desirable traits from different plants, then genetic diversity and trait combination are improved, but genetic non-uniformity and unpredictable performance occur

Engineering Contradiction:
Improvetrait combinationVSAvoidperformance predictability
Core Design Contradiction:
Adaptability or versatilityVSReliability

Solution Approach 1:

The breeding process is segmented into distinct phases: creating diverse hybrids through cross-pollination, then separating and selecting uniform inbred lines through self-pollination. This segmentation allows the system to first achieve trait combination diversity, then achieve performance predictability in the inbred generation.

Inventive Principle:
Principle #1Segmentation

Solution Approach 2:

Instead of using cross-pollination to achieve uniformity (conventional approach), the patent uses self-pollination to achieve uniformity from diverse parents. The inversion of the pollination method resolves the contradiction by producing uniform inbreds that can be reliably used for hybrid production.

Inventive Principle:
Principle #13The other way round (Inversion)

2Reliability

If self-pollination is used to produce uniform inbred plants, then genetic uniformity and breeding stability are improved, but loss of genetic diversity and trait combination capability occur

Engineering Contradiction:
Improvebreeding stabilityVSAvoidgenetic diversity
Core Design Contradiction:
ReliabilityVSAdaptability or versatility

Solution Approach 1:

Cross-pollination and trait combination are performed as a preliminary action before self-pollination. Diverse hybrids are created first, then self-pollinated to produce uniform inbreds. This preliminary diverse crossing resolves the contradiction by establishing genetic diversity before the uniformity phase.

Inventive Principle:
Principle #10Preliminary action

Solution Approach 2:

The F1 hybrid generation acts as an intermediary between diverse parental lines and uniform inbred lines. The F1 hybrids carry the genetic diversity of their parents while being uniform enough to produce stable inbred progeny through self-pollination, thus mediating between diversity and stability requirements.

Inventive Principle:
Principle #24Intermediary (Mediator)

3Reliability

If extensive breeding programs and multiple generations of selection are used to develop superior inbreds, then desired traits and uniformity are improved, but time consumption and breeding complexity increase

Engineering Contradiction:
Improvetrait stabilityVSAvoidbreeding cycle duration
Core Design Contradiction:
ReliabilityVSLoss of time

Solution Approach 1:

Traditional mechanical field-based selection and breeding operations are replaced with molecular marker-assisted selection and genomic selection methods. This substitution dramatically reduces the time required to identify and select superior inbred lines while maintaining trait stability.

Inventive Principle:
Principle #28Mechanics substitution (Replace mechanical system)

Solution Approach 2:

The breeding program utilizes changes in genetic parameters through marker-assisted selection and genomic information to accelerate trait fixation. By using molecular markers to track desired traits, the number of generations required to achieve stable inbreds is reduced while maintaining breeding reliability.

Inventive Principle:
Principle #35Parameter changes

4Measurement precision

If traditional field-based selection methods are used to evaluate hybrid performance, then phenotypic selection accuracy is improved, but evaluation time and resource requirements increase

Engineering Contradiction:
Improvephenotypic selection accuracyVSAvoidevaluation period
Core Design Contradiction:
Measurement precisionVSLoss of time

Solution Approach 1:

Traditional field-based phenotypic evaluation is supplemented or replaced with molecular marker analysis and genomic prediction models. These methods provide rapid, accurate assessment of breeding values without requiring extended field trial periods, thus reducing evaluation time while maintaining or improving selection accuracy.

Inventive Principle:
Principle #28Mechanics substitution (Replace mechanical system)

Solution Approach 2:

Molecular markers and genomic profiles create a copy of the genetic information that can be evaluated rapidly without growing the plants to maturity. This copying approach allows parallel evaluation of multiple candidates simultaneously, dramatically reducing the time required for phenotypic selection accuracy assessment.

Inventive Principle:
Principle #26Copying

Data Source

PatentUS9307726B2Plants and seeds of corn variety CV382815
Publication Date: 2016.04.12 MONSANTO TECHNOLOGY LLC

AI summary

According to the invention, there is provided seed and plants of the corn variety designated CV382815. The invention thus relates to the plants, seeds and tissue cultures of the variety CV382815, and to methods for producing a corn plant produced by crossing a corn plant of variety CV382815 with itself or with another corn plant, such as a plant of another variety. The invention further relates to corn seeds and plants produced by crossing plants of variety CV382815 with plants of another variety, such as another inbred line. The invention further relates to the inbred and hybrid genetic complements of plants of variety CV382815.