Corn Variety CV405228 Stable Inbred Line Development

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Solution Overview

Problem

Corn breeding aims to combine desirable traits like high yield, disease resistance, and uniformity, but existing methods struggle to produce stable inbred plants with predictable performance due to genetic non-uniformity from cross-pollination, leading to unpredictable hybrid traits.

Innovation Solution

The development of the corn variety CV405228, which includes a cytoplasmic or nuclear factor for male sterility or self-incompatibility, along with genetic modifications such as single locus conversions for traits like herbicide tolerance and disease resistance, and the use of tissue cultures to regenerate plants with consistent morphological and physiological characteristics.

Engineering Contradictions & Design Principles

VSEngineering Contradiction Analysis

1Adaptability or versatility

If cross-pollination is used in corn breeding, then genetic diversity and trait combination are improved, but genetic uniformity and predictability of hybrid traits deteriorate

Engineering Contradiction:
Improvegenetic diversityVSAvoidgenetic uniformity
Core Design Contradiction:
Adaptability or versatilityVSStability of the object's composition

Solution Approach 1:

The breeding population is divided into separate inbred lines that are homozygous at almost all gene loci. Each inbred line is genetically uniform and stable, but when crossed together they produce hybrid progeny with genetic diversity and heterosis. This segmentation allows controlled combination of desirable traits while maintaining predictability through known parental genotypes.

Inventive Principle:
Principle #1Segmentation

Solution Approach 2:

The invention changes the genetic parameter from heterozygous to homozygous state through repeated self-pollination over multiple generations. This creates inbred plants with fixed gene loci that serve as stable parental lines. The homozygous parameter ensures uniformity within each line while allowing predictable hybrid outcomes when lines are crossed.

Inventive Principle:
Principle #35Parameter changes

2Stability of the object's composition

If self-pollination is used to develop inbred lines, then genetic uniformity and stability are improved, but time required for breeding and trait combination are increased

Engineering Contradiction:
Improvegenetic stabilityVSAvoidbreeding time
Core Design Contradiction:
Stability of the object's compositionVSLoss of time

Solution Approach 1:

Inbred parental lines are developed in advance through repeated self-pollination and selection before the hybrid breeding program begins. These pre-developed homozygous lines serve as ready-to-use parental material, eliminating the need to develop inbreds during the hybrid breeding process. This preliminary action saves significant time in the overall breeding program.

Inventive Principle:
Principle #10Preliminary action

Solution Approach 2:

The breeding program maintains continuous useful action by having multiple inbred lines developed and ready for crossing. Rather than developing one inbred line at a time sequentially, the program has a library of pre-developed inbreds that can be crossed in various combinations, allowing parallel development of multiple hybrids simultaneously.

Inventive Principle:
Principle #20Continuity of useful action

3Manufacturing precision

If multiple generations of selfing are performed to achieve homozygosity, then uniformity of inbred progeny is improved, but productivity and speed of variety development are reduced

Engineering Contradiction:
Improveuniformity of inbred progenyVSAvoidspeed of variety development
Core Design Contradiction:
Manufacturing precisionVSProductivity

Solution Approach 1:

The breeding program segments the development process into independent inbred line development modules. Each inbred line is developed separately through selfing to homozygosity, ensuring uniformity. Once multiple inbred lines are developed, they can be crossed in various combinations to produce hybrids, allowing parallel productivity in the hybrid generation without compromising the uniformity achieved in the inbred parental lines.

Inventive Principle:
Principle #1Segmentation

Data Source

PatentUS11229176B1Plants and seeds of corn variety CV405228
Publication Date: 2022.01.25 MONSANTO TECHNOLOGY LLC

AI summary

According to the invention, there is provided seed and plants of the corn variety designated CV405228. The invention thus relates to the plants, seeds and tissue cultures of the variety CV405228, and to methods for producing a corn plant produced by crossing a corn plant of variety CV405228 with itself or with another corn plant, such as a plant of another variety. The invention further relates to corn seeds and plants produced by crossing plants of variety CV405228 with plants of another variety, such as another inbred line. The invention further relates to the inbred and hybrid genetic complements of plants of variety CV405228.