Corn Variety CV454023 Breeding for Genetic Predictability

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Solution Overview

Problem

Corn breeding aims to combine desirable traits like yield, disease resistance, and uniformity, but existing methods struggle to develop stable inbred plants with predictable performance due to genetic non-uniformity from cross-pollination, leading to unpredictable hybrid traits.

Innovation Solution

The development of the corn variety CV454023, which includes a cytoplasmic or nuclear factor for male sterility and specific genetic loci conferring traits like herbicide tolerance and disease resistance, along with a process for producing inbred and hybrid seeds through controlled pollination and backcrossing techniques.

Engineering Contradictions & Design Principles

VSEngineering Contradiction Analysis

1Adaptability or versatility

If cross-pollination is used to combine desirable traits from different plants, then genetic diversity and trait combination are improved, but genetic non-uniformity and performance predictability deteriorate

Engineering Contradiction:
Improvetrait combinationVSAvoidperformance predictability
Core Design Contradiction:
Adaptability or versatilityVSReliability

Solution Approach 1:

The breeding process is segmented into distinct phases: developing homozygous inbred lines through self-pollination, then crossing these inbreds to create uniform F1 hybrids. This segmentation allows each phase to serve its specific purpose - inbreeding for genetic uniformity, crossing for trait combination - thereby resolving the contradiction between diversity and predictability.

Inventive Principle:
Principle #1Segmentation

Solution Approach 2:

Homozygous inbred plants are developed through multiple generations of self-pollination and selection before the actual crossing step. This preliminary action ensures that the parental lines are genetically uniform and stable, so that when they are crossed, the F1 hybrids exhibit uniform and predictable performance, eliminating the non-uniformity problem.

Inventive Principle:
Principle #10Preliminary action

2Stability of the object's composition

If self-pollination is used to develop homozygous inbred plants, then genetic uniformity is improved, but the need for physical emasculation and manual pollination increases

Engineering Contradiction:
Improvegenetic uniformityVSAvoidemasculation process
Core Design Contradiction:
Stability of the object's compositionVSDevice complexity

Solution Approach 1:

The plant performs self-pollination automatically through its own pollen transfer mechanism, eliminating the need for manual emasculation and pollination operations. The natural self-pollinating behavior of the plant serves the breeder's need for homozygosity without requiring complex manual intervention, thus resolving the contradiction between uniformity and process complexity.

Inventive Principle:
Principle #25Self-service

3Stability of the object's composition

If multiple generations of selfing and selection are performed to develop inbreds, then homozygosity is improved, but breeding time and productivity are reduced

Engineering Contradiction:
ImprovehomozygosityVSAvoidbreeding cycle duration
Core Design Contradiction:
Stability of the object's compositionVSLoss of time

Solution Approach 1:

The most time-consuming selfing and selection operations are performed as preliminary actions to establish homozygous inbred lines once. After this initial investment, the F1 hybrids can be produced through a single crossing event, which is much faster. The preliminary development of uniform parents ensures that subsequent hybrid production is rapid and predictable, resolving the time loss contradiction.

Inventive Principle:
Principle #10Preliminary action

4Reliability

If physical emasculation is used to prevent self-pollination, then cross-pollination control is improved, but labor requirements and operational complexity increase

Engineering Contradiction:
Improvecross-pollination controlVSAvoidmanual pollination requirement
Core Design Contradiction:
ReliabilityVSEase of operation

Solution Approach 1:

The plant's natural self-pollinating mechanism is harnessed to achieve cross-pollination control without manual emasculation. By allowing the plant to self-pollinate to create homozygous inbreds, then using these inbreds in controlled crosses, the system achieves reliable cross-pollination control while eliminating the labor-intensive manual emasculation process, resolving the contradiction between control reliability and operational ease.

Inventive Principle:
Principle #25Self-service

Data Source

PatentUS9072241B2Plants and seeds of corn variety CV454023
Publication Date: 2015.07.07 MONSANTO TECHNOLOGY LLC

AI summary

According to the invention, there is provided seed and plants of the corn variety designated CV454023. The invention thus relates to the plants, seeds and tissue cultures of the variety CV454023, and to methods for producing a corn plant produced by crossing a corn plant of variety CV454023 with itself or with another corn plant, such as a plant of another variety. The invention further relates to corn seeds and plants produced by crossing plants of variety CV454023 with plants of another variety, such as another inbred line. The invention further relates to the inbred and hybrid genetic complements of plants of variety CV454023.