Corn Variety CV516216 Breeding for Genetic Uniformity

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Solution Overview

Problem

Current corn breeding techniques face challenges in developing uniform corn hybrids with desirable traits such as high yield, disease resistance, and uniform germination, as they often result in unpredictable performance due to genetic non-uniformity among hybrid plants.

Innovation Solution

The development of the corn variety CV516216, which includes a cytoplasmically-inherited male sterility trait and the ability to introduce specific genetic loci for traits like herbicide tolerance, insect resistance, and modified metabolism, along with a process for producing hybrid seeds by crossing inbred plants to ensure genetic uniformity and desired characteristics.

Engineering Contradictions & Design Principles

VSEngineering Contradiction Analysis

1Adaptability or versatility

If conventional corn breeding techniques are used to develop hybrids, then various desirable traits can be combined in a single variety, but the resulting hybrid plants exhibit genetic non-uniformity leading to unpredictable performance

Engineering Contradiction:
Improvecombination of desirable traitsVSAvoidperformance predictability
Core Design Contradiction:
Adaptability or versatilityVSReliability

Solution Approach 1:

The breeding process is segmented into distinct stages: developing homozygous inbred parental lines through self-pollination and selection, then crossing these standardized inbreds to produce uniform F1 hybrids. This segmentation ensures each parental line is genetically stable before combination, resolving the contradiction between trait versatility and performance predictability.

Inventive Principle:
Principle #1Segmentation

Solution Approach 2:

The invention emphasizes creating homogeneous inbred parental lines that are homozygous at almost all gene loci through multiple generations of self-pollination and selection. This homogeneity in the parental lines ensures uniform F1 hybrid progeny, directly addressing the genetic non-uniformity problem while maintaining the ability to combine multiple desirable traits.

Inventive Principle:
Principle #33Homogeneity

2Stability of the object's composition

If self-pollination is used to develop homozygous inbred plants, then uniform true-breeding progeny are produced, but the breeding process requires extensive time and generations of selection

Engineering Contradiction:
Improvegenetic uniformityVSAvoidbreeding cycle duration
Core Design Contradiction:
Stability of the object's compositionVSLoss of time

Solution Approach 1:

The invention applies preliminary action by extensively developing and stabilizing inbred parental lines through multiple generations of self-pollination and selection before the actual hybrid crossing. This preliminary groundwork ensures genetic uniformity is established in advance, allowing the F1 hybrid generation to immediately exhibit consistent, predictable performance without requiring additional selection time.

Inventive Principle:
Principle #10Preliminary action

Solution Approach 2:

The self-pollination process is leveraged as a self-service mechanism where the plant naturally performs pollination to itself, facilitating the development of homozygous inbred lines. This self-service approach maintains genetic uniformity through automatic selection while reducing the need for complex external intervention, though it still requires multiple generations to achieve complete homozygosity.

Inventive Principle:
Principle #25Self-service

3Adaptability or versatility

If cross-pollination is used to produce hybrid plants, then genetic diversity is increased, but the resulting population lacks uniformity and shows unpredictable performance

Engineering Contradiction:
Improvegenetic diversityVSAvoidpopulation uniformity
Core Design Contradiction:
Adaptability or versatilityVSStability of the object's composition

Solution Approach 1:

The invention resolves this contradiction by ensuring both parental inbred lines are highly homogeneous (homozygous at almost all loci) before crossing. This preliminary homogenization allows the cross-pollination to produce F1 hybrids that are genetically uniform and predictable, while still benefiting from the genetic diversity introduced by combining two different inbred lines with complementary desirable traits.

Inventive Principle:
Principle #33Homogeneity

Solution Approach 2:

The breeding program is segmented into separate phases: first developing standardized homogeneous inbred lines through self-pollination, then using controlled cross-pollination between these standardized lines. This segmentation allows genetic diversity to be systematically introduced at the crossing stage while maintaining population uniformity through the use of genetically stable parental lines.

Inventive Principle:
Principle #1Segmentation

Data Source

PatentUS8247665B2Plants and seeds of corn variety CV516216
Publication Date: 2012.08.21 MONSANTO TECHNOLOGY LLC

AI summary

According to the invention, there is provided seed and plants of the corn variety designated CV516216. The invention thus relates to the plants, seeds and tissue cultures of the variety CV516216, and to methods for producing a corn plant produced by crossing a corn plant of variety CV516216 with itself or with another corn plant, such as a plant of another variety. The invention further relates to corn seeds and plants produced by crossing plants of variety CV516216 with plants of another variety, such as another inbred line. The invention further relates to the inbred and hybrid genetic complements of plants of variety CV516216.