Corn Variety CV595836 Cytoplasmic Male Sterility Hybrid Breeding

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Solution Overview

Problem

Corn breeding aims to combine desirable traits like higher yield, disease resistance, and uniformity, but existing methods struggle to develop stable inbred plants with predictable performance due to genetic non-uniformity in hybrid plants.

Innovation Solution

The development of the corn variety CV595836, which includes a cytoplasmic or nuclear factor for male sterility and genetic modifications such as herbicide tolerance, insect resistance, and specific metabolic traits, along with a process for producing hybrid seeds by controlled cross-pollination to ensure uniformity and desirable characteristics.

Engineering Contradictions & Design Principles

VSEngineering Contradiction Analysis

1Stability of the object's composition

If self-pollination is used to develop uniform inbred plants, then genetic uniformity is improved, but breeding time and generation cycles are extended

Engineering Contradiction:
Improvegenetic uniformityVSAvoidbreeding time
Core Design Contradiction:
Stability of the object's compositionVSLoss of time

Solution Approach 1:

The patent applies preliminary action by using cytoplasmic male sterility to prevent self-pollination before it can occur, thereby forcing cross-pollination and hybrid seed production. This eliminates the need for lengthy self-pollination breeding cycles while achieving the desired genetic uniformity through controlled hybridization of predetermined inbred lines.

Inventive Principle:
Principle #10Preliminary action

Solution Approach 2:

The patent uses cytoplasmic male sterility as an intermediary mechanism to control pollination. The sterile cytoplasm acts as a mediator that prevents unwanted self-pollination while allowing controlled cross-pollination between specific inbred lines, thereby accelerating the breeding process while maintaining genetic uniformity.

Inventive Principle:
Principle #24Intermediary (Mediator)

2Adaptability or versatility

If cross-pollination is used to produce hybrid plants, then genetic diversity and trait combination are improved, but genetic uniformity and performance predictability deteriorate

Engineering Contradiction:
Improvetrait combinationVSAvoidgenetic uniformity
Core Design Contradiction:
Adaptability or versatilityVSStability of the object's composition

Solution Approach 1:

The patent segments the breeding process into distinct phases: first developing pure inbred lines through self-pollination to achieve genetic uniformity, then using cytoplasmic male sterility to control cross-pollination between specific inbred lines. This segmentation allows each phase to optimize for its specific goal, achieving both genetic uniformity within lines and desirable trait combination in hybrids.

Inventive Principle:
Principle #1Segmentation

Solution Approach 2:

The patent changes the pollination parameter from uncontrolled cross-pollination to controlled cross-pollination using cytoplasmic male sterility. By changing the sterility parameter through cytoplasmic factors, the system achieves consistent, predictable hybrid production with uniform genetic composition while maintaining the benefits of cross-pollination for trait combination.

Inventive Principle:
Principle #35Parameter changes

3Ease of operation

If manual emasculation is used to prevent self-pollination, then control over cross-pollination is improved, but labor requirements and production cost increase

Engineering Contradiction:
Improvepollination controlVSAvoidlabor requirements
Core Design Contradiction:
Ease of operationVSDevice complexity

Solution Approach 1:

The patent applies self-service by using cytoplasmic male sterility to enable plants to automatically prevent self-pollination without human intervention. The sterile cytoplasm causes the plant to produce non-functional pollen, eliminating the need for manual emasculation and significantly reducing labor requirements while maintaining complete control over cross-pollination.

Inventive Principle:
Principle #25Self-service

Solution Approach 2:

The patent replaces the mechanical system of manual emasculation with a biological system based on cytoplasmic male sterility. Instead of physically removing anthers through labor-intensive manual operations, the system uses cytoplasmic factors to naturally prevent pollen formation, thereby eliminating the need for manual intervention while maintaining pollination control.

Inventive Principle:
Principle #28Mechanics substitution (Replace mechanical system)

4Reliability

If extensive breeding programs are implemented to develop superior inbreds, then desirable characteristics are improved, but program complexity and resource requirements increase

Engineering Contradiction:
Improvedesirable characteristicsVSAvoidprogram complexity
Core Design Contradiction:
ReliabilityVSDevice complexity

Solution Approach 1:

The patent applies preliminary action by pre-developing and maintaining pure inbred lines with known genetic characteristics before hybrid production. This preliminary development phase allows breeders to select and characterize inbred lines for specific desirable traits, then use cytoplasmic male sterility to efficiently produce hybrids with predictable performance, thereby reducing overall program complexity.

Inventive Principle:
Principle #10Preliminary action

Solution Approach 2:

The patent uses cytoplasmic male sterility as an intermediary to simplify breeding program management. The sterile cytoplasm acts as a reliable marker and control mechanism that facilitates systematic hybrid production between predetermined inbred lines, reducing program complexity by providing a clear, manageable framework for trait combination and hybrid seed production.

Inventive Principle:
Principle #24Intermediary (Mediator)

Data Source

PatentUS20190357488A1Plants and seeds of corn variety CV595836
Publication Date: 2019.11.28 MONSANTO TECHNOLOGY LLC

AI summary

According to the invention, there is provided seed and plants of the corn variety designated CV595836. The invention thus relates to the plants, seeds and tissue cultures of the variety CV595836, and to methods for producing a corn plant produced by crossing a corn plant of variety CV595836 with itself or with another corn plant, such as a plant of another variety. The invention further relates to corn seeds and plants produced by crossing plants of variety CV595836 with plants of another variety, such as another inbred line. The invention further relates to the inbred and hybrid genetic complements of plants of variety CV595836.