Corn Variety CV652404 Breeding for Genetic Uniformity

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Solution Overview

Problem

Corn breeding aims to combine desirable traits like yield, disease resistance, and uniformity, but existing methods struggle to develop stable inbred plants with predictable performance due to genetic non-uniformity from cross-pollination, leading to unpredictable hybrid traits.

Innovation Solution

The development of the corn variety CV652404, which includes a cytoplasmic or nuclear factor for male sterility to prevent self-pollination, along with specific genetic loci for traits like herbicide tolerance and disease resistance, and the use of tissue cultures to regenerate plants with consistent physiological and morphological characteristics.

Engineering Contradictions & Design Principles

VSEngineering Contradiction Analysis

1Adaptability or versatility

If cross-pollination is used to combine desirable traits from different plants, then genetic diversity and trait combination are improved, but genetic non-uniformity and unpredictable performance occur

Engineering Contradiction:
Improvetrait combinationVSAvoidperformance predictability
Core Design Contradiction:
Adaptability or versatilityVSReliability

Solution Approach 1:

The breeding process is segmented into distinct phases: creating diverse hybrid populations through cross-pollination, then separating and selecting uniform individuals through self-pollination and selection. This segmentation allows the system to first achieve genetic diversity for trait combination, then achieve uniformity for predictable performance by isolating and replicating successful genotypes.

Inventive Principle:
Principle #1Segmentation

Solution Approach 2:

The patent changes the pollination parameter from cross-pollination to self-pollination during the selection phase. By switching from cross-pollination (which creates diversity) to self-pollination (which creates uniformity), the system transitions from generating genetic variation to stabilizing specific traits, achieving both diversity in the population level and uniformity in the selected lines.

Inventive Principle:
Principle #35Parameter changes

2Stability of the object's composition

If self-pollination is used to create uniform inbred plants, then genetic uniformity and breeding stability are improved, but loss of genetic diversity occurs

Engineering Contradiction:
Improvegenetic uniformityVSAvoidgenetic diversity
Core Design Contradiction:
Stability of the object's compositionVSAdaptability or versatility

Solution Approach 1:

Cross-pollination and trait combination are performed as preliminary actions before self-pollination. The diverse hybrid population is created first through cross-pollination of plants with different desirable traits, then self-pollination is applied to stabilize and uniformize the selected genotypes. This preliminary cross-pollination ensures genetic diversity is established before uniformity is achieved through selfing.

Inventive Principle:
Principle #10Preliminary action

Solution Approach 2:

The breeding system dynamically switches between cross-pollination mode (for diversity generation) and self-pollination mode (for uniformity stabilization). This dynamic transition allows the system to adapt its pollination strategy based on the breeding objective: diversity when needed, uniformity when stability is required, achieving both goals at different temporal stages.

Inventive Principle:
Principle #15Dynamics

3Reliability

If multiple breeding generations are used to develop stable inbreds, then breeding stability and trait uniformity are improved, but breeding time and complexity increase

Engineering Contradiction:
Improvebreeding stabilityVSAvoidbreeding cycle duration
Core Design Contradiction:
ReliabilityVSLoss of time

Solution Approach 1:

Selection feedback is applied continuously throughout the breeding generations. Plants are evaluated for desired traits at each generation, and only those meeting the criteria are selected for the next generation. This feedback mechanism accelerates the convergence toward stable inbreds by eliminating non-conforming individuals early, reducing the number of generations needed compared to blind self-pollination.

Inventive Principle:
Principle #23Feedback

Solution Approach 2:

The breeding program applies different strategies to different plant lines based on their specific characteristics and desired traits. Lines showing rapid convergence to stability are advanced faster, while those requiring more refinement continue through additional generations. This localized, differentiated approach optimizes the breeding cycle duration for each line based on its specific genetic composition and trait expression.

Inventive Principle:
Principle #3Local quality

Data Source

PatentUS8604311B2Plants and seeds of corn variety CV652404
Publication Date: 2013.12.10 MONSANTO TECHNOLOGY LLC

AI summary

According to the invention, there is provided seed and plants of the corn variety designated CV652404. The invention thus relates to the plants, seeds and tissue cultures of the variety CV652404, and to methods for producing a corn plant produced by crossing a corn plant of variety CV652404 with itself or with another corn plant, such as a plant of another variety. The invention further relates to corn seeds and plants produced by crossing plants of variety CV652404 with plants of another variety, such as another inbred line. The invention further relates to the inbred and hybrid genetic complements of plants of variety CV652404.