Cyanine Dye Derivatives for Higher-Accuracy Nucleic Acid Sequencing
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Solution Overview
Problem
There is a need for improved cyanine derivatives that can be effectively utilized in sequencing applications to enhance the performance of sequencing methods.
Innovation Solution
The development of cyanine derivatives, including compounds of Formula (I), (II), or (III), their ionic derivatives, isomers, and salts, which can be used as fluorescent dyes in sequencing methods, potentially enhancing the efficiency and accuracy of sequencing processes.
Engineering Contradictions & Design Principles
Engineering Contradiction Analysis
1Measurement precision
If conventional cyanine dyes are used in sequencing applications, then basic fluorescent detection is achieved, but sequencing accuracy and efficiency are insufficient
Solution Approach 1:
The patent modifies the chemical structure of cyanine dyes by introducing specific substituents (such as sulfur-containing groups, fluorinated alkyl chains, and cyclic structures) to alter their photophysical properties. These parameter changes in molecular structure result in improved fluorescent characteristics including higher quantum yield, enhanced photostability, and optimized emission wavelengths, thereby resolving the contradiction between measurement precision and reliability in sequencing applications
2Productivity
If existing fluorescent dyes are used, then sequencing can be performed, but the efficiency and accuracy of sequencing processes are limited
Solution Approach 1:
The patent creates composite fluorescent dye structures by combining cyanine cores with various functional moieties (such as benzothiazole, carbazole, and fluorinated groups) to produce hybrid molecules that exhibit synergistic effects. These composite structures simultaneously enhance the fluorescent brightness and photostability (improving accuracy) while maintaining appropriate size and binding characteristics (improving efficiency), thus resolving the contradiction between productivity and measurement precision
Applied Scientific Principles
This section explains which scientific principles are used to turn an abstract innovation direction into a practical engineering solution.
Function Achieved in This Case
The cyanine derivatives provide improved performance in sequencing applications by offering enhanced fluorescent properties, thereby improving the accuracy and efficiency of sequencing methods.
Implementation Method 1
Cyanine dyes are particularly popular fluorophores and are widely used in many biological applications
Data Source
AI summary
The present disclosure provides a compound of Formula (I), (II), or (III):an ionic derivative thereof, an isomer thereof, or a salt thereof. The present disclosure also provides conjugates of the compounds, and methods of using the compounds and the conjugates. The disclosure also provides the use of the compounds and conjugates in methods of sequencing nucleic acids.